PCR/qPCR䪮助唬崵

鍒Ɀ倰呩

2

湡
䔶

呋劥ꅷ꧋

‡ RNA呋劥

‡ 㹔庛⥂㶸❡ㅷ

呍ꃑ䲿》

‡ 呍ꃑ䲿》

‡ DNA呋劥㢅椚

‡ RNA呋劥㢅椚

呍ꃑ䪈㟞

‡ Taqꃍ

‡ 鷟鲮䔶ꃍ

‡ 䒸暟ծ䱲ꛏ

‡ Proligo

‡ PCR幑⸈⵫

‡ PCR霚⵫渱

ꃍ

Oligo &

Proligo

1$3幑⸈⵫

呍ꃑ䪈㟞

ThermaSTOP™

ThermaGO™

欫蝲焦

䒸暟

䱲ꛏ

❇熧ꃀ腂⽀⡤

Jumpstart™Taqꃍ

M-MLV鷟鲮䔶ꃍ

eAMV鷟鲮䔶ꃍ

3/\"呋劥 呋劥㹔庛⥂㶸

DNAstable ®

RNAstable ®

DEPC

RNAꃍ䫈ⵖ⵫

RNAlater®

呋劥ꅷ꧋

崵䎸

Magprep®煂棟

跗涯ꃍK

呍ꃑ䲿》

workflow

呋劥ꅷ꧋

搋焫ꃑ✳⛩ꂾ DEPC

僽♧猫欽✵抣崞RNaseꃍ䎇㼆宐ⴔㄤpH

併䠭涸⻊㷖ⵖㅷկ㖈155˫傞㸐㖈宐很巊⚥〳ⴔ鍒䧭⛩ꃫㄤ✳

孻⻊焫կ搋焫ꃑ✳⛩ꂾ㖈PCR⿿䎾⚥欽✵㢅椚⿡猌㶩宐⟄

꣭⡛✫RNA鄄RNase꣭鍒涸굥ꤗկ

Protector RNase䫈ⵖ⵫ 餗〿R2520

絆ⴔProtector RNase䫈ⵖ⵫ 8NjO

⪰㶸巊20mM

HEPES-KOH50mM KCl8mM✳炻蔼祪ꃫ50%欥屘

v/v

pH 7.6䊩〸կ

⻊㷖䒭O(COOC2H5)2

ⴔ㶩ꆀ162.14

㺙䏞1.101 g/mL 25 °C勵⟝♴

鵘鳕倰䒭ⱺ㗌鵘鳕

⥂㶸庛䏞2-8°C

暵ⵆ䲿ꄀDEPC㼜很鍒♧❈㝕俱獴巊㐼♶Ⱟ㺂翸薅⛩掜㺂

㐼䎾⢪欽梯槴獴巊㐼կ

RNA呋劥

RNAꃍ䫈ⵖ⵫

DEPC搋焫ꃑ✳⛩ꂾ

餗〿⿺⺫鄳

餗〿 ❡ㅷ錞呔

D5758-5ML

D5758-25ML

D5758-50ML

D5758-100ML

D5758-5ML

D5758-25ML

D5758-50ML

D5758-100ML

3

㖈cDNAざ䧭⿿䎾ծRT-PCR ⠛絡掚䗄梠⟉ㄤqPCR禹絡

⚥

㥵LightCycler®⟉

ծ⡤㢪鲮䔶/缺霖禹絡ծRNAꃍ⥂

䫡㹊낉ծ⡤㢪RNAざ䧭⚥⥂䫡mRNA

㖈⡤㢪氻嫫㢕ⵖ鵂玐⚥⥂䫡氻嫫RNA

㖈RNAⴔ猌紱⻊鵂玐⚥䫈ⵖRNAꃍ崞䚍

鳇⸔ⵖ㢊偽RNAꃍ涸䫒⡤

ざ䧭欽✵⾲⡙勇❜涸RNA䱲ꛏ

䎾欽

‡

‡

‡

‡

‡

掚珘㹁䚍涸RNase䫈ⵖ⵫⽰⢪⢪欽掚珘㹁䚍鷟鲮䔶ꃍ 㥵

Transcriptor鷟鲮䔶ꃍ

涸勵⟝♴Protector RNase䫈ⵖ

⵫➠⥂䭯珘㹁կProtector RNase䫈ⵖ⵫㖈pH 5.0荛9.0勵

⟝♴ㄤ+25°C荛+55°C涸庛䏞♴鿪Ⱘ剣崞䚍 +60°C傞➠

⥂殆鿈ⴔ崞䚍

կ鼙⯝ぐ猫RNAⴔ區䎾欽⚥涸䎁䪓⽰⢪⼮

넞ⵌ叻ⲥ崽䏞涸16⦔➠⥂䭯姻䌢佪腊կ䭯絯䲿⣘넞紱䏞❡

暟嫦♧䪠妃涸❡ㅷ鿪鵳遤⚚呔餘䱽崨玐絑㹊傞㹁ꆀ

RT-PCR瘝叻ⲥ䪮助唬崵烁⥂♶ゎ呍ꃑⰻⴗꃍծ呍祪呍ꃑ

ꃍ䧴ⴗⶶ崞䚍կ

暵挿

RNAlater®僽♧猫宐湱涸偽嫫䚍絆絉⪰㶸霚⵫〳㖈劢ⲇⲏ

涸呋劥⚥䘯鸟瑬鷳絆絉⟄㖈⾲⡙㼆絈脅RNA鵳遤珘㹁ㄤ⥂

䫡կ絆絉㗌㖈佐꧋た用⽰嵵尵㖈RNAlater⚥鵳遤⪰㶸罜♶

⠔䰀㹲RNA涸餘ꆀ䧴侨ꆀկRNAlater⯝⿡✫㼆絆絉呋劥用⽰

鵳遤㢅椚䧴ⲇⲏ㖈巊宆⚥欽✵た絯㢅椚涸꨽銳կRNAlater㖈

37°Cծ25°Cㄤ4°C⥂㶸絆絉⚥涸RNAⴔⵆꟋ鴪1㣔ծ♧ワㄤ

♧⚡剢կ絆絉鵮〳㖈-20°C⥂㶸刿Ɤ傞ꢂկ

⢪欽倰岁⪰㶸涸絆絉呋ㅷⴗⶶ䧭㖈荛㼱♧⚡絶䏞♳㼭✵0.5

cm䎇嵵尵㖈5⦔⡤獤涸RNAlater⚥䔲ⲥ㢊㥩꨽銳ⴔ猌

RNA傞〳➢RNAlater⚥》ⴀ絆絉䎇䭽撑⫸⴮佐꧋ⵌ涸絆絉

♧呋鵳遤㢅椚կ㼆✵絈脅⪰㶸〳㖈⸈Ⰶ5-10⦔⡤獤涸

RNAlater⛓⵸⢪欽㼱ꆀ涸PBS欽✵ꅾ䝧小巤涸絈脅կ㖈ⵖ㢊

RNA⛓⵸㼜絈脅鵳遤小巤䎇䒙♳幡կ

RNAlater®

呋劥⥂㶸

랾⯘欰ㄐ猰㷖荝⸂✵⚹䝠䌄勻刿梠⥂涸剏➿❡ㅷ霪❡ㅷ⚁

⚹䲿넞腊彂佪桧罜霃雦կBiomatrica®❡ㅷ⢪㷖助歲蚋暟

䒓〄ㄤⴔ㶩霏倗곭㚖涸灇瑕➃プ腊㢿珘㹁Ɤ劍珘㹁㖑鵘鷐

ㄤ㶸⪰欰暟呋ㅷ䎇腊㢿⟄ꬋ䌢⡛䐢涸䧭劥蜦䖤㸤侮罜䘯鸟

涸呋ㅷ㔐佐կBiomatrica®涸 SampleMatrix®僽♧고⚁剣呍

䗱䪮助〳㖈㹔庛♴珘㹁ㄤ⥂䫡欰暟勞俱罜♶⠔꣭鍒կ

DNAstable ® ⢪䝠腊㢿㖈㹔庛♴⥂㶸ծ㶸⪰ㄤ鵘鳕巊⡤ㄤ

liquid-to-dry䕎䒭涸紱⻊DNA呋ㅷկ㸐Ⱘ剣넞抳崞䚍㖈暵

婋庛䏞♴Ⱘ剣掚珘㹁䚍䎇⚂腊㢿㢅椚넞崽䏞DNAկ〫꨽⸈

宐⽰〳㖈侨ⴔꛦⰻ➢䎁斕㛇餘⚥㔐佐կ䝠涸DNA呋ㅷ〳用

⽰欽✵♴康ⴔ區偽꨽⟣⡦Ⱖ➭紱⻊姿낢կ

4

RNAstable®僽♧猫杝暵涸⪰㶸➝餘〳㖈㹔庛♴⥂㶸RNA呋

ㅷ侨剢կ⢪欽RNAstable®䝠〳⟄鸑鵂皍⽀涸ꅾ倝宐⻊⽰

〫꨽⸈宐⤑〳㸤Ⰼ䛪㢕䝠涸RNA呋ㅷկⱄ宐⻊涸呋ㅷ〳用⽰

⢪欽偽꨽鵳♧姿紱⻊կ嫦⚡盗䧴匣䨾ゎ剣涸RNAstable®僽

⡲⚹盗䧴㶰䏀鿈涸嶃㽻罜䲿⣘涸〳⥂䫡淼⯘荛䗎⯘紩涸

RNAկ

⢪欽倰岁⥂㶸㖈RNAstable⚥涸RNA〳鸑鵂⸈Ⰶ宐䧴宐湱

綕Ȿ巊㢕蔼կ偽꨽鵳♧姿紱⻊呋ㅷ⤑〳湬䱹欽✵♴康䎾欽կ

RNAstable®

㼜 10-100ul DEPC 㢅椚鵂涸宐䧴宐湱綕Ȿ巊湬䱹⸈Ⰶ荛

RNAstable ⚥䎁斕涸呋ㅷկ

㖈㹔庛㷱肫15ⴔꛦ⟄㹊梡⯎ⴔ涸宐⻊կ

鸑鵂綕䢨オ䩧㼜呋ㅷ鵳遤幊⺣կ鼙⯝❡欰孞岙կ宐⻊涸呋

ㅷ〳湬䱹欽✵♴康涸䎾欽կ

劢⢪欽涸宐⻊呋ㅷ㖈4°C䧴㹔庛剒㢴⪰㶸3㣔կ呋ㅷ

鵮 〳ⲇⲏ⪰㶸 -20°C 䧴-80°C

刿Ɤ傞ꢂկ㖈ゎ剣

RNAstable®涸宐⚥ⱄ宐⻊涸呋ㅷ⻊〳ꅾ倝䎁斕罜䎇♶

⠔鸣䧭呋ㅷ珘㹁佪桧꣭⡛կ♶䲀虛ꅾ㢕霪鵂玐馄鵂♲妃կ

1.

2.

3.

4.

鸑鵂綕䢨䰤䩧㼜呋ㅷ幊⺣ 鼙⯝❡欰孞岙

կ

㼜劢銻渷涸呋ㅷ鵳遤䎁斕կ㼆✵⡤獤㼭✵20ul涸⤑䰦䒭瑟

孞䎁斕➑꨽㼜盗䧴96㶰匣㖈馄ⲙ〵⚥䒓渷鵂㢹կ㼆✵⡤

獤20ul䧴꨽銳⸈鸟䎁斕〳⢪欽♶⸈掚涸溫瑟崽綫㐼 䎁

斕傞ꢂ〳⿬罌♴倰涸邍1

կ

㼜䎁斕⵫⺫鄳㖈⾲㨤郍⚥䎇⸈掚㺙㼓կ⛲〳㼜䎁斕涸呋ㅷ

㖈㹔庛♴⪰㶸㖈䎁斕涸⪰㶸厷⚥կRNAstable®㼆庛䏞ㄤ

弪䏞併䠭㔔姼㖈㹔庛 15-25°C

♴㼜呋ㅷ⥂㶸㖈꣈惐涸

梠㞯⚥荛Ⱒꅾ銳կ䒊雳涸弪䏞宐䎂㼭✵30湱㼆弪䏞կ

3.

4.

5.

RNAstable 呋ㅷ䎁斕⿺⪰㶸

崵㹁呋ㅷ⚥紱⻊RNA涸ꆀ䎇雦皾⸈ⰆRNAstable®㶰䧴

盗⚥涸ꆀկ㖈⸈ⰆRNAstable®⛓⵸䎾㼜RNAꅾ䝧✵

DEPC㢅椚涸宐⚥կ

ぢゎ剣RNAstable®涸㶰䧴盗涸⚥㣛湬䱹綕䢨⸈Ⰶ剒㢴

100ul涸RNA呋ㅷկ⚹✫㹊梡葻㥩涸呋ㅷ⥂䫡ㄤ䛪㢕佪

卓⸈Ⰶⵌ嫦㶰⚥欽✵瑟孞䎁斕涸剒絊呋ㅷ⡤獤䎾䔲㼭✵

20u嫦㶰䧴盗⚥♶銳⸈Ⰶ馄鵂100ul涸宐կ

1.

2.

呋劥㢕很

⥂㶸㖈RNAstable®⚥涸RNA〳鸑鵂⸈Ⰶ宐䧴宐湱綕Ȿ巊

㢕蔼կ偽꨽鵳♧姿紱⻊呋ㅷ⤑〳湬䱹欽✵♴康䎾欽կ

㼜 10-100ul DEPC 㢅椚鵂涸宐䧴宐湱綕Ȿ巊湬䱹⸈Ⰶ荛

RNAstable® ⚥䎁斕涸呋ㅷկ

㖈㹔庛㷱肫 15 ⴔꛦ⟄㹊梡⯎ⴔ涸宐⻊կ

鸑鵂綕䢨オ䩧㼜呋ㅷ鵳遤幊⺣կ鼙⯝❡欰孞岙կ宐⻊涸呋ㅷ

〳湬䱹欽✵♴康涸䎾欽կ

劢⢪欽涸宐⻊呋ㅷ㖈4°C䧴㹔庛剒㢴⪰㶸3㣔կ呋ㅷ

鵮 〳 ⲇⲏ⪰㶸 -20°C 䧴-80°C

刿Ɤ傞ꢂկ㖈ゎ剣

RNAstable®涸宐⚥ⱄ宐⻊涸呋ㅷ⻊〳ꅾ倝䎁斕罜䎇♶

⠔鸣䧭呋ㅷ珘㹁佪桧꣭⡛կ♶䲀虛ꅾ㢕霪鵂玐馄鵂♲妃կ

1.

2.

3.

4.

5.

邍 
ˊ
ꅷ欽溫瑟崽綫㐼涸䎁斕傞ꢂ

呋ㅷ⡤獤 NjO

10–20

20–30

30–100

䎁斕傞ꢂ KUV7XEHV 盗

0.5

1.0

1.5

❡ㅷ䲽鶣

62001-046 DNAgard® Tissue 100 mL bottle, for room temp preservation of DNA

93021-001 DNAstable® Tube kit, for room temp preservation of DNA

90021-001 DNAstable® 96-well plate, for room temp preservation of DNA

53091-016 DNAstable® Plus 2 ml, for room temp preservation of DNA

52091-026 DNAstable® Plus 10 mL bottle, for room temp preservation of DNA

93221-001 RNAstable® Tube Kit, for room temp preservation of RNA

90220-001 NAstable® 96-well plate, for room temp preservation of RNA

53201-013 RNAstable® LD 2 ml, for room temp preservation of RNA

52201-013 RNAstable® LD 10mL bottle, for room temp preservation of RNA

62501-046 DNAgard® Blood 100mL bottle, for room temp preservation of DNA

62501-026 DNAgard® Blood 10mL bottle, for room temp preservation of DNA

93027-027 DNAstable® Blood tubes, for room temp preservation of DNA

93020-017 DNAstable® Blood Trial Kit, for room temp preservation of DNA

52001-027 DNAstable® Blood LD 10 mL bottle, for room temp preservation of DNA

63301-011 PCRboost® 1 mL bottle, for improved PCR performance

63301-001 PCRboost® Trial Kit, for improved PCR performance

93421-013 RNAconcentrator™ Tube Kit, for concentration of RNA samples

63401-001 STRboost® 50 rxn, for multiplexing PCR samples

餗〿

❡ㅷ⴩邍

5

呍ꃑ䲿》

跗涯ꃍ,

꣭鍒跗涯餘꣈姺呍ꃑ꣭鍒

跗涯ꃍK㾩✵䎛靶⚏孽ꃑ跗涯ꃍ僽鸑欽涸꣭鍒跗涯涸ꃍ〳

⟄⿡ꤑ呍ꃑ呋ㅷ⚥涸跗涯䎁䪓⢾㥵DNasesㄤRNases

⥂䫡呍ꃑ呋ㅷ♶鄄꣭鍒䎛岌欽✵➃絆絉䧴絈脅涸呍ꃑ䲿

》կ

ㅷ餘⥂霆遤⚌叻勍⧩䖤⥌饆կ랾⯘跗涯ꃍK涸⚚呔欰❡䊨

蒌⥂霆✫ꃍ涸珘㹁䚍Ⱘ剣⠏䒗涸〳ꬑ䚍ㄤ紱䏞刿㥩㖑忘駈

霏倗涸銳宠կ

㉁ㅷꃍ⚥劢唬崵ⵌ呍ꃑ涸婎殆䨾⟄暵ⵆ鷓ざた絯PCRㄤ

RT-PCR㼆䪈㟞❡ㅷ鵳遤ⴔ區կ

⺫鄳룅Ⰼ磋劣ծ巊⡤㣐㼭⺫鄳㢴猫錞呔〳鷥կ

❡ㅷ䲽鶣

㖈DNAㄤRNA涸ⴔ猌紱⻊鵂玐⚥欽✵呍ꃑꃍ涸跗涯宐鍒

㣟崞

⿡ꤑ♸꣉猌㶩跗涯餘㥵很蝒ꃍㄤRnase A絕ざ涸ⰻ嫫稇

㢅椚瀖蹙⺫㙵涸絆絉ⴗ晙⟄凐ꪪ䫒⾲䧴呍ꃑ絕ざ⡙挿欽✵

䫒⡤䧴䱲ꛏ叻雵

䎾欽

‡

‡

‡

珘㹁涸㹊낉絕卓〳ꬑ涸ㅷ餘ㄤ葻㥩涸䪠ꢂ䊵䱽ⵖ僽ⴔ♶

䒓涸跗涯ꃍ,❡ㅷ絕ざ✫⯓鵳涸欰❡䊨蒌䪠ꢂ♧荝䚍

絑鵂⚚呔涸ⴔ區崵霚

㟞⸈湡涸㛇㔔䪈㟞❡暟⚚呔涸唬崵烁⥂偽呍ꃑꃍ涸婎殆

剣佪涸ꥬ猌⡛䭡餍垷匣

⠏挿

‡

‡

‡

磋劣跗涯ꃍK

괃蒀: 涯蒀

朐䙖: 䎁磋

ꃍ崞䚍

KHPRJORELQffS+ffƒ&ffl•P$QVRQ8PJ

ꃍ嫱崞FDOFRQSURWHLQffl•P$QVRQ8PJ

DNases (Nicking activity; pBR 322; 6 h; 37 °C):

not detectable

RNases (RNA; 2 h; 37°C): not detectable

餗〿 朐䙖 ⺫鄳錞呔

1.24568.0100

1.24568.0500

1.24568.0010

1.24568.9100

539480-100MGCN

539480-1GMCN

100mg

500mg

10g

100g

100mg

1g

䎁磋

䎁磋

䎁磋

䎁磋

䎁磋

䎁磋

巊⡤跗涯ꃍK

괃蒀: 涯蒀

朐䙖: 巊⡤

⺫鄳10ml1L

ꃍ崞䚍 (hemoglobin; pH 7.5; 37 °C):

•P$QVRQ8PO

崽䏞ffl•PROO

DNases (Nicking activity; pBR 322; 6 h; 37 °C):

not detectable

RNases (RNA; 2 h; 37°C): not detectable

餗〿 朐䙖 ⺫鄳錞呔

1.07393.0010

1.07393.1000

10ml

1L

巊⡤

巊⡤

6

㼜Ꝇ꩓❵ㄤ稇⥝껑䗎椕䎾欽✵欰暟ⴔ猌ծ欰暟紱⻊䧴欰暟唬

崵僽㛇✵Ꝇ꩓❵ㄤ稇ㄤ欰暟稇叻雵涸ⴔ㶩Ⱘ剣䖎넞涸❵ㄤ⸂կ

Ꝇ꩓❵ㄤ稇♸欰暟稇涸絕ざ⸂䔂 Kd=10-15M

鵯雮ⴔ猌ծ

紱⻊ծ唬崵欰暟稇⥝껑涸ⴔ㶩腊㢿㖈♶ず涸pH⧩ 3-10

ծ綕

Ȿ巊 0.01-3.0M渮崽䏞

ծ⟄⿺ゎ剣霽㥵SDS䧴ち庛-20瘝牙

㘻⵫涸勵⟝♴鵳遤♶「⟣⡦䕧ㆇկꝆ꩓❵ㄤ稇⥝껑涸煂䚍

䗎椕⚹ⴔ猌䧴紱⻊ぐ猫欰暟稇叻雵ⴔ㶩 ⺫䭍過巊ծ過幡ծ絆

䰘灇瑕邍僈ꤑ✫邍꬗⚹炊繿㛇⥝껑涸紵碛煂곾磜邍꬗⚹

續㛇⥝껑涸煂䚍䗎椕ず呋鷓欽✵PCR❡暟ծ餘磜DNA⟄⿺絈

蝒DNA涸䲿》ㄤⴔ猌1կEstapor®馄곡煂䚍䗎椕禹⴩❡ㅷ

㖈DNA䲿》䎾欽倰꬗獤稡✫鳅㢴涸絑낉㖈磜䖈ծ邍꬗ㄤ煂

ゎꆀ倰꬗鿪腊絛䝠⠍㢴鷥䭊կ

續㛇煂䚍䗎椕䲿》DNA涸⡲欽劼椚僽㖈翸ざ暟⟄⿺꣉猌㶩渮

涸Ⱏず⡲欽♴䒸〄涸煂棟/DNA涸翸꧋⡲欽կ鸑䌢朐䙖♴涸

DNAⴔ㶩ッ偽錞ⴭ⽷刼朐䙖䔲綕Ȿ巊⚥ず傞⸈Ⰶ♧㹁崽䏞

❡ㅷ 湬䖈ĞN 煂䚍꜈涰ⴔ嫱翸ざ暟 餗〿

M1-020/50

M1-030/40

M1-050/20

M1-070/40

M1-070/60

M1-130/12

M1-180/12

M1-180/20

M1-200/20

EM1-100/20

EM1-100/30

EM1-100/40

0.160-0.240

0.251-0.400

0.500-0.700

0.700-1.300

0.700-1.300

0.700-1.200

0.900-1.300

0.800-1.200

1.700-2.700

0.900-1.800

0.900-1.800

0.900-1.800

＞50

40-60

16-25

35-45

55-65

10-15

10-15

16-25

20-40

15-25

26-35

36-50

80380073

80380000

39574001

23709083

23711081

39490084

39432084

80380560

80380001

39451080

39394082

23710087

翸薅⛩掜

翸薅⛩掜

翸薅⛩掜

翸薅⛩掜

翸薅⛩掜

翸薅⛩掜

翸薅⛩掜

PS/DVB

翸薅⛩掜

翸薅⛩掜

翸薅⛩掜

翸薅⛩掜

Ꝇ꩓❵ㄤ稇⥝껑䗎椕

Estapor®馄곡煂䚍續㛇䗎椕

絉ㄤ굹ㅷ瘝♶ず勻彂涸䫒⾲ծ䫒⡤䧴呍ꃑ

䲿⣘✫♧⚡珘㹁涸

㔿湱կ湡⵸Ꝇ꩓❵ㄤ稇⥝껑涸䗎椕䊺欽✵唬崵鷓⡤

aptamer

-跗涯⿿䎾涸歏⻊㷖倰岁♳ 9

⟄⿺罣歌孻銯卌ꆄ랕

蒀袕蟎椕蝒涸䘯鸟唬崵♳ 2

կ

Ⱖ㸐䎾欽絈蝒崽綫ծ➃䧴⸓暟絈脅ⴔ猌ծ跗涯餘絆㷖ծ

PCR ❡暟紱⻊կ

A A A A

AT

A A A A T T T T A A A AT T T T

A A A A

T T T T

A A A A

T T T T

T T T T T T T T

Bio-EstaporꝆ꩓❵ㄤ稇䗎椕䎾欽✵mRNA 欰暟紱⻊

呋ㅷ

➃絈脅

⸈Ⰶ鄬鍒

綕Ȿ巊

⸈Ⰶ BioEstapor 涸Ꝇ꩓

❵ㄤ稇⥝껑煂棟

 ㄤ欰暟稇

叻雵涸㻑翸dT

⸈Ⰶ DNA ꃍ 煂ⴔ猌 峤嶷 煂ⴔ猌 mRNA 峤膴

涸PEGㄤNaCl傞DNAⴔ㶩涸⽷刼朐䙖⠔㝋綫鷶幯䕎䧭刿

⚹珘㹁涸椕朐絕匬կ霪椕朐絕匬涸䫔〜鿈ⴔⳜ⛖⚹歏⚥䚍

➑ⶥ♴10%涸劢䫔〜⽷刼鿈ⴔ䌄餏歏կ㔔姼㖈鵯呋涸넞渮

梠㞯♴煂䚍䗎椕邍꬗♸DNAⴔ㶩❡欰꫽歏㾓誋鸑鵂孲ꝶ

⡲欽❡欰絕ざկ㖈㸤䧭DNAⴔ㶩涸エ꣡姿낢た煂䚍䗎椕ⱄ

絑鵂ꃫ峤ㄤ⡛渮綕Ȿ巊涸峤嶷⽰〳剒絊蜦䖤湡叻DNAⴔ

㶩կⰦ⚥⧩䖤岤䠑涸僽ꛏ㼆♶ずⴔ㶩ꆀ㣐㼭涸DNAⴔ㶩

䧮⟌䎾霪㼆PEG涸ⴔ㶩ꆀծPEG崽䏞ծNaCl崽䏞⟄⿺た絯峤

膴姿낢瘝鵳遤䊨蒌♳涸⠏⻊23կ

♳鶣♧❈䗎椕䊺䎛岌欽✵⯝氋唬崵ㄤⰦ➭고湡2-6

EStapor®馄곡煂䚍䗎椕涸歏Ꞥ撑晙⿬罌䗎椕M1-070/40

7

MagPrep® Silica 煂곾磜

絑Ⱙ涸呍ꃑ DNAㄤRNA

ⴔ猌倰岁⣜饆✵崽䏞唑䏞猌䗱ㄤ薅

ꂛ- 守⟬蟋》կ鵯⚙猫倰岁署傞ծ餩⸂Ⳝ⛖♶腊荈⸓⻊鵮

꨽⢪欽⽭ꤗ⻊㷖ㅷկ㥵➚ぐ㣐Ⱆ぀姻荝⸂✵䒓〄刿⸈㸝Ⰼ

涸鍒Ɀ倰呩䲿넞呍ꃑ紱⻊涸鸑ꆀկ

MagPrep® Silica煂곾磜 㥵㕃1䨾爙

姻⚹忘駈鵯♧꨽宠罜䒓

〄鸑鵂煂ⴔ猌涸倰岁欽✵荈⸓⻊呍ꃑ紱⻊倰呩⚥կ

MagPrep® Silica煂곾磜䌄剣姻歏蚚僒✵エ꣡䌄餏歏蚚涸呍

ꃑ⚺銳欽✵➢➃過幡䧴過崰呋ㅷ⚥䲿》DNAㄤRNA ⢾㥵➢

過巊呋劥⚥唬崵HIV䧴HBV氻嫫㛇㔔絆涸呋ㅷⵖ㢊

կ♧菚罜

鎊㼜呋ㅷ♸絕ざ綕Ȿ巊ㄤMagPrep® Silica煂곾磜幊ざ呍

ꃑ♸煂곾磜絕ざた絑鵂侨妃峤嶷♸煂㖞䯲蜦峤膴♴勻涸

呍ꃑ⽰〳鸑鵂PCR䧴Ⱖ㸐暵㹁倰岁勻唬崵氻嫫暵䒗DNA䧴

RNAկ湡⵸MagPrep® Silica⡲⚹⾲俱䊺䎛岌欽✵霏倗遤⚌

涸넞鸑ꆀ荈⸓⻊禹絡⚥կ

MagPrep® Silica煂곾磜僽歋邍꬗⺫醙满♧㽻炊涸煂꜈瀤絆䧭կ

鵯❈䝧嵥㖈綕Ȿ巊(50mM MES, pH 5.5)⚥涸랱蒀곾磜䎂㖲

磜䖈⚹100nm(鸑䌢⚹50–200nm)կ㼭磜䖈涸煂곾磜䭆剣刿

㣐涸邍꬗獤鵯㼆腊や➢㣐ꆀ呋劥⚥䧭⸆䲿》䗎ꆀ涸湡涸ⴔ

㶩荛Ⱒꅾ銳կ姼㢪磜䖈㼭涸煂곾磜〳⥂䭯Ⳝⴔꛦ涸䝧嵥朐

䙖鵯⛲⚹湡涸ⴔ㶩涸絕ざ䲿⣘✫⯎酕涸傞ꢂկ

歋✵煂ゎꆀ넞鴪95%煂곾磜䖎㺂僒㖈煂㖞⚥鴽鸟ⴔ猌կ鸑

䌢䞔ⲃ♴
煂㖞䯲蜦⿿䎾㖈2ⴔꛦⰻ⽰〳㸤䧭կ

煂ⴔ猌䪮助雮ぐ㣐㹊낉㹔腊㢿㖈♧㣔傞ꢂⰻ㹊梡䧭⼪♳♰涸

呍ꃑ呋劥荈⸓⻊紱⻊կ姼㢪霪䪮助㖈唬崵霽㥵絈脅磦䲿》

暟䧴碫⡃굹ㅷ磦⺣崰暟瘝㢕勇呋ㅷ傞⛲Ⱘ剣⠏⸷կ

㕃 1. 歏Ꞥ撑晙♴ MagPrep® 炊磜㶩涸磜䖈ㄤ兪⡤絕匬

MagPrep® Silica煂곾磜涸暟椚㾩䚍

‡ 湬䖈~100nm

‡ 絆ⴔ95%涸煂꜈瀤

‡ 邍꬗獤16-22 m2/mg 偽㶰

‡ エ꣡腊⸂10µg DNA䧴RNA/mg

皍➝

‡ 磜㶩崽䏞5%(50 mg/ml)

‡ ⺫鄳錞呔1 ml、5ml、10 ml ⿺㹐䨪雧ⵖ㣐⺫鄳

‡ 綕Ȿ巊磜㶩䝧嵥㖈宐⚥

‡ ⥂㶸⥂㶸✵4-8˫

MagPrep® 炊磜㶩杝暵⸆腊

‡ 腊➢Ⰼ過⚥エ꣡涯絈脅呍ꃑ

‡ 腊➢欰Ɤ㛇餘⚥エ꣡絈蝒呍ꃑ

‡ 腊➢過崰⚥エ꣡氻嫫呍ꃑ

‡ 腊㖈넞猌巊ㄤꃑ䚍勵⟝♴エ꣡呍ꃑ

‡ 〳⢪欽宐䚍䧴ゎ⛩ꃫ涸綕Ȿ巊峤嶷

MagPrep® 炊磜㶩涸䎾欽

‡ エ꣡絈蝒

‡ エ꣡氻嫫

‡ エ꣡涯絈脅

‡ ⴔ猌過巊⚥涸ⰌDNA

‡ ⴔ猌絈蝒⚥涸ⰌDNA

‡ ⴔ猌氻嫫DNA/RNA

‡ 紱⻊PCR晙媯

‡ ⿡ꤑ崵䎸⿿䎾⚥涸厩俱

‡ ⴔ猌餘磜DNA

MagPrep® Silica煂곾磜⢪欽銳挿

䧮⟌〳䲿⣘ 3 猫 MagPrep® Silica 煂곾磜“LS”㛇炄㘗

“MS” 㘗ㄤ “HS” 㘗կ“LS” 㘗エ꣡ RNA 佪卓㥩㖈暵㹁勵⟝

♴⛲腊エ꣡ DNAկ“HS” 㘗鷥䭊䚍エ꣡ DNAկ“MS” 㘗〳ず傞

ꅾ䝧

MagPrep® Silica⚹50mg/ml랱蒀곾磜涸䝧嵥巊㖈⢪欽⵸

꨽銳ꅾ䝧幊⺣կ䌢錛涸ꅾ䝧倰岁⺫䭍♳♴䮷虹ծ嶴偒瘝倰

岁կⰦ⚥䌢錛剣佪涸僽⢪欽䌄剣1ml tip㣢涸獴巊㐼⿿㢕オ

䩧կ㼆✵刿㣐⡤獤涸㺂㐼〳縨✵忎櫕䧴䶔䎯幊ざⳜⴔꛦ

ꅾ䝧た꨽欽聘滚唬叅㺂㐼ⰻ䝧嵥巊涸婎⡮곾磜կ꨽銳⧩䖤岤

䠑涸僽㖈⢪欽Ⰼ荈⸓禹絡傞峤嶷姿낢⚥涸ꅾ䝧〳⟄鸑鵂䗄

梠煂㖞⢪煂곾磜屠❜剏倰ぢ獴⸓կ

ⴔ猌

MagPrep® Silica 煂곾磜90% ⟄♳䧭⟧⚹煂꜈瀤㔔姼僒✵

㖈煂㖞⚥䘯鸟ⴔ猌կ歋♶ず⾊㹻䲿⣘涸絟㣐㢴侨煂卹⚹宕煂

⡤⢪欽佪卓椚䟝կ鸑䌢䔲⡤獤⚹1ml傞䯲蜦⿿䎾署傞㼱✵

5ⴔꛦկ㼆✵ⴔ猌刿㣐㺂ꆀ䧴⡤獤ⴭ꨽銳刿㢴涸傞ꢂ⢪欽

刿㣐䧴刿䔂涸煂꜈կ煂ⴔ猌䪮助㼆넞鸑ꆀ荈⸓⻊禹絡涸呋ㅷ

㢅椚䧴㢅椚霽㥵絈脅磦䲿》暟䧴굹ㅷ碫磦⺣崰瘝㢕勇呋劥傞

ꬋ䌢剣⠏⸷կ搬罜㥵ꬋ䗳곢⢪欽姻❜ⴔ猌傞⛲〳鸑鵂瀊

傞ꢂ涸猌䗱ⴔ猌㼭⚂
ꅾ
涸곾磜կ

❡ㅷぜ獦 ⺫鄳錞呔* 崽䏞 餗〿

MagPrep® Silica LS

1 ml

50 mg/ml

1.01193-0001

50 ml 1.01193-0050

MagPrep® Silica MS

1 ml

50 mg/ml

1.01644-0001

25ml 1.01644-0025

MagPrep® Silica HS

1 ml

50 mg/ml

1.01899-0001

50 ml 1.01899-0050

〳呏䰘㹐䨪꨽宠䲿⣘刿㣐涸⺫鄳錞呔կ

㷱肫

湡叻ⴔ㶩㖈絕ざ涸鵂玐⚥鸑䌢尵剣䗳銳⢪煂곾磜⥂䭯䝧嵥

㔔⚹侮⚡⿿䎾〫꨽銳侨猲罜煂곾磜涸䝧嵥朐䙖⠔䭯絯Ⳝⴔ

ꛦկ㼆✵Ⱖ➭涸㷱肫姿낢㥵卓꨽銳㔿㹁⼮庛ⴭ䒊雳⢪欽

꩎⸓㐼䧴幊ざ㐼կ

絕ざ

DNAㄤRNA㖈♶ず絕ざ綕Ȿ巊⚥鿪〳絕ざⵌMagPrep ®

Silica 煂곾磜涸邍꬗կ剣⚙猫䌢錛涸⻊㷖ㅷ⠔⤛鵳絕ざⴔⵆ

⚹넞崽䏞涸猌巊⵫䧴ꃑ䚍絕ざ綕Ȿ巊կ綕Ȿ巊涸䧭ⴔ㖈乼⡲

姿낢⚥⠔⴩ⴀ⟄⣘⿬罌կ䔲呋ㅷゎ剣⿡㘻⵫傞 㥵SDS

넞崽

䏞猌巊⢾㥵5M涸炻宊ꃑ胟♸⛩ꃫ䧴䒗⚋ꃫ涸幊ざ暟⛲〳⡲⚹

絕ざ綕Ȿ巊կ

峤嶷

DNA䧴RNA♸煂곾磜絕ざた〳欽㢴猫宐䚍峤嶷綕Ȿ巊幡峤

湡叻ⴔ㶩♶⠔㖈峤嶷鵂玐⚥峤膴♴勻կ宐䚍峤嶷綕Ȿ巊剣❈

䞔ⲃ♴⠔殜⨊ꃑ䚍䧴ゎ剣鷓ꆀ⛩ꃫկ

峤膴

峤膴綕Ȿ巊涸pH⧩鸑䌢넞✵8.0⚂⿿䎾鵂玐꨽銳⼮庛կⰦ

➭勵⟝䧴綕Ȿ巊⛲僽〳遤涸կ⢪欽歌ꃀ腂峤膴ꬋ䌢剣佪⚂〳

㖈㹔庛勵⟝♴鵳遤♶鵂♴康䎾欽곭㚖鳅㼱կ峤膴佪桧〳㼜

ꅾ䝧た涸煂곾磜⸈Ⰶⵌ楹腒祪⚥鸑鵂歏岷唬崵կ歏峤膴涸呍

ꃑ〳鸑鵂EB厩俱䧴Ⱖ㸐倰岁唬崵կ

エ꣡ DNA ㄤ RNAկ欽䨪〳呏䰘呍ꃑ紱⻊鵂玐⚥涸絕ざծ峤嶷

勵⟝⟄⿺呋劥碫㘗涸♶ず鷥䭊鷓欽䵨ꂁկ

8

낉霆侨䰘
ⴔⵆ➢0.5ծ1ծ2ㄤ4ml過崰⚥紱⻊䗄梠DNA(䊩)ⴔⵆ》2ul紱⻊涸DNA鵳遤qPCR唬崵5S rRNA㛇㔔涸湱㼆ゎꆀ

5S RNAꥥ满呋劥ꆀ㟞⸈ッ絁䚍㟞Ɤ邍僈Ⱖ刿㥩涸♧荝䚍ㄤ䖤桧(⚥)qPCR唬崵5S rRNA㛇㔔涸絁䚍䏞(〸)qPCR⿿䎾⚥

㟞⸈垷匣ꆀ♶䕧ㆇ䧴Ct⧩꣭⡛邍僈蜦䖤涸DNA紱䏞넞♶ゎPCR䫈ⵖ暟կ

GenElute™過崰/過幡康猌䗄梠DNA⚥ꆀ紱⻊霚⵫渱㛇✵猌䗱叙㽻區岁欽✵➢1mL~4mL倝됮䧴ⲇⲏ過崰/過幡呋ㅷ⚥倰⤑ծ

䘯鸟ծ〳ꬑ㖑䲿》ぐ猫㣐㼭涸康猌䗄梠DNA(cfc-DNA)紱⻊䨾䖤涸過崰/過幡cfc-DNA㼜㖈峤膴巊⚥鵳遤峤膴⟄Ⱟ㺂⟣⡦♴康

䎾欽⺫䭍PCRծ㹊傞㹁ꆀPCRծ歌㛇⻊併䠭涸PCRㄤSouthern⽪鶺ⴔ區ծ䗎꣋⴩ㄤNGS瘝կ

9

GenElute™ 過崰/過幡康猌䗄梠DNA⚥ꆀ紱⻊

霚⵫渱DNB600)

*HQ(OXWHŒ㛇㔔絆DNA䲿》霚⵫渱

䘯鸟蜦䖤넞餘ꆀDNA

䘯鸟넞佪〳㖈40-45ⴔꛦⰻ紱⻊ⴀぐ猫㣐♴涸䗄梠DNA

넞䏞紱⻊DNA餘ꆀ넞♶ゎ䫈ⵖ暟

䎛岌Ⱟ㺂Ⱟ㺂⟣⡦♴康䎾欽

⢪欽抳崞呋ㅷ饰㨤ꆀ〳1-4ml峤膴⡤獤〳50-100ul

❡ㅷ⴩邍

RNB600

RNB700

RNB500

DNB600

NA2010

NA2020

G1N9604

BLD9601

RDP300

G1N70

DNB100

DNB300

G2N70

RDP100

DNB400

RDP200

RNB400

DNB200

NA2110

GenElute™ 過崰/過幡RNA⚥ꆀ紱⻊霚⵫渱

GenElute™ 過崰/過幡RNA㣐ꆀ紱⻊霚⵫渱

GenElute™ 過崰/過幡RNA㼭ꆀ紱⻊霚⵫渱

GenElute™ 過崰/過幡康猌䗄梠DNA⚥ꆀ紱⻊霚⵫渱

GenElute™ 過巊㛇㔔絆DNA霚⵫渱

GenElute™ 過巊㛇㔔絆DNA霚⵫渱

GenElute™ 96㶰絆絉/㛇㔔絆DNA紱⻊霚⵫渱

GenElute™ 96㶰過巊㛇㔔絆DNA紱⻊霚⵫渱

GenElute™ RNA/DNA/跗涯餘紱⻊霚⵫渱

GenElute™ ㇄✌⸓暟㛇㔔絆DNA㼭ꆀⵖ㢊霚⵫渱

GenElute™ 㕼㡝DNAⴔ猌霚⵫渱

GenElute™ 㽶巊康猌DNA㼭ꆀ紱⻊霚⵫渱

GenElute™ 嗃暟㛇㔔絆DNA㼭ꆀⵖ㢊霚⵫渱

GenElute™ 宐⡤RNA/DNA紱⻊霚⵫渱NjP

GenElute™ 瀖蹙⺫㙵(FFPE)絆絉DNA紱⻊霚⵫渱

GenElute™ 瀖蹙⺫㙵(FFPE)絆絉RNA/DNA紱⻊霚⵫渱

GenElute™ 瀖蹙⺫㙵(FFPE)絆絉RNA紱⻊霚⵫渱

GenElute™ 礋⤑DNAⴔ猌霚⵫渱

GenElute™ 絈蝒㛇㔔絆DNA霚⵫渱

❡ㅷ⴩邍

餗〿 ❡ㅷ䲽鶣

10

Benzonase®呍ꃑꃍ

Benzonase®呍ꃑꃍ僽♧妴䎛靶涸呍ꃑꃍ⽰⡲欽✵DNA⛲

⡲欽✵RNA㖈氻嫫㛇㔔絆崵䎸⚥〳⟄꣭鍒㺋⚺絈脅涸呍

ꃑ罜♶⡲欽✵氻嫫呍ꃑ剒鷓涸ꃍ崞pH⚹8.0-9.2կ㸐〳

㼜呍ꃑ㸤Ⰼ嶊⻊⚹Ɤ䏞⚹3荛5⚡焦㛇涸Ļ⽀熧ꃑ劣畮㻑呍薗

ꃑ鵯㼆✵氻嫫瘝䗎欰暟㛇㔔崵䎸䎾欽僽ꬋ䌢椚䟝涸鷥䭊կ

㖈㛇㔔崵䎸㹊낉⚥㔔⚹䪈㟞❡暟䎸⴩꨽銳溫㹊䚍䧮⟌偽

岁㖈⿿䎾⡤禹⚥幑⸈UNG⢪欽Benzonase®呍ꃑꃍ㢅椚孞

很脸⛲僽♶ꝡ涸鷥䭊կ

呍ꃑ䲿》鳇俱

岤 : ⢪欽 EDTA 轌ざꆄ㾩猌㶩〳㹊梡〳鷟䫈ⵖկ♶〳鷟涸抣崞

〫腊㖈暵婋勵⟝♴ 70°C ♴ 100 mM NaOH 㖈 30 ⴔꛦⰻ

㸤䧭կ

餗〿 䲽鶣

71206

70746

E8263

E1014

Benzonase® Nuclease HC, Purity > 99%

Benzonase® Nuclease, Purity > 90%

Benzonase®呍ꃑꃍ馄紱•XQLWVNj/

•ffiffi6'63$*(UHFRPELQDQW

Benzonase®呍ꃑꃍ•XQLWVNj/

•ffi6'63$*(UHFRPELQDQW

餗〿 䲽鶣

L4509

L3771

G4505

G6639

G3272

G9277

L7386

L2524

L4025

R4875

R6315

R5000

D5025

4716728001

C8511

P7000

D9779

L4509

EDS

C7901

95577

95621

C9426

SDS

SDS

渮ꃑ胟

䒗炻宊ꃑ胟

渮ꃑ胟

炻炻宊ꃑ胟

很袕椕蝒ꃍ

很絈脅ꃍ

很絈脅ꃍ

Rnase A

Rnase A

Rnase A

Dnase I

ꅾ絆DNase I

絆絉跗涯ꃍC

胊跗涯ꃍ

DTT

SDS

EDTA

Chelex-100

Chelex-100

Chelex-100

CHAPS宐ざ暟

11

DNTP-10⽀杝涸㔋盗 dATPdGTPdCTPdTTP崽䏞

10mM

DNTP-100 ⽀杝涸㔋盗 dATPdGTPdCTPdTTP崽䏞

100mM

D7295: dNTP mix崽䏞10mM

71004-M崽䏞10mM

1.

2.

3.

4.

䚍腊⠏葻涸䫒⡤㼓꡻taqꃍ傋㖈剒㼭⻊ꬋ暵䒗䚍䪈㟞ず傞

䲿넞湡叻❡ꆀկ♧傉⿿䎾庛䏞鴪ⵌ70°CTaq DNA翸ざꃍ崞

䚍䛪㢕䨾䖤PCR儑爙ⴀ刿넞涸暵䒗䚍ㄤ❡桧կ

⢪欽岤䠑✲고PCR⿿䎾⚥dNTP崽䏞♧菚㖈50։Nj

mol/Lկ岤䠑4猫dNTP涸崽䏞銳湱瘝㥵卓⟣⡦♧猫dNTP涸

崽䏞鵂⡛կ❡欰ꝡꂁ涸Ⳝ桧㼜⠔㣐㣐㟞㣐㥵卓dNTP㣐侮⡤

崽䏞鵂⡛ⴭ⠔꣭⡛PCR⿿䎾⚥湡叻❡暟涸ꆀ㥵卓dNTPꆀ

鵂넞ⴭ⠔♸Mg2+絕ざ꣭⡛Mg2+涸剣佪崽䏞կ

䲀虛 Buffer 1. 100mM Tris-HCl (pH 8.3); 500 mM

KCl; 60 mM (NH4)2SO4; 1% Triton X-100; 0.01%

BSA.

䲀虛幑⸈⵫DMSOծ欫蝲焦

♶䲀虛幑⸈⵫欥屘

⺫ゎ♲⚡絆ⴔJumpStart Taq ꃍ10X Buffer〳鷥䭊僽

やゎ MgCl2ㄤ 25mM MgCl2 欽✵⠏⻊կ

呍ꃑ䪈㟞

-XPS6WDUWŒ7DT'1$翸ざꃍ

G173

%XIIHU ⚥♶ゎ Mg2+D4184 餗〿⿺⺫鄳錞呔

餗〿 ⺫鄳錞呔

D4184-50UN

D4184-250UN

D4184-1.5KU

20ul

100ul

600ul

崽䏞

2.5units/ul

2.5units/ul

2.5units/ul

欽ꆀ 絊崽䏞

ZNj/

Nj/

[Nj/

Nj/

Nj/

Nj/

Nj/

\\Nj/

Nj/

]Nj/

Nj/

-

1x

1.5-5mM

Nj0

Nj0

Nj0

Nj0

Nj0

XQLWVNj/

SJNj/

霚⵫

宐

10x PCR buffer

MgCl2

10 mM dATP

10 mM dCTP

10 mM dGTP

10 mM dTTP

Primers

JumpStart Taq DNA Polymerase

10ng Template DNA

Totaol reaction volume

%XIIHU ⚥♶ゎ Mg2+D9307 餗〿⿺⺫鄳錞呔

餗〿 ⺫鄳錞呔

D9307-50UN

D9307-250UN

D9307-1.5KU

20ul

100ul

600ul

崽䏞

2.5units/ul

2.5units/ul

2.5units/ul

㼆✵꨽銳Ⲹ㼱ꬋ暵䒗䚍䪈㟞涸PCR䪈㟞

欽✵㢴ꅾPCR

Ⲹ㼱䒸暟✳翸⡤

䎾欽 fl

幑⸈ꆀ

⺫鄳ㄤ崽䏞

⺫鄳 fl

朐䙖巊⡤

暵䚍掚゗⸓

崽䏞XQLWVNj/

괃蒀偽蒀鷳僈

鵘鳕倰䒭弪ⱺ

⥂㶸庛䏞íƒ&

‡

‡

‡

掚゗⸓ꃍ崞⻊傞ꢂ瀊

ꃍ䪈㟞偽⨊㥩䚍

湡涸㛇㔔❡ꆀ넞

暵挿 fl

‡

‡

‡

PCR ざ䧭 DNA Ꝇ⾲俱涸 4 猫焦㛇կ

⺫鄳  ⺫鄳  ⺫鄳 

餗〿M1302

䚍餘

朐䙖巊⡤

괃蒀偽蒀

鵘鳕倰䒭䎁ⱺ

⥂㶸庛䏞-20°C

M-MLV 鷟鲮䔶ꃍ➢㖈餘磜♳邍鴪M-MLV pol 㛇㔔涸

Escherichia coli⚥紱⻊䨾䖤腊㢿⸈鸟dNTP䕎䧭熧ꃑ✳ꂾ

ꝶ➢罜⪶⻊RNA垷匣侮ざⵌ✽邉DNA⚥կM-MLV鷟鲮䔶ꃍ

僽♧猫㼜⽀ꝆRNAծDNA䧴RNA-DNA勇ざ⡤ ⵄ欽䒸暟

ざ䧭

✽邉DNAꝆ涸DNA翸ざꃍկM-MLV〳欽✵♧姿岁RT-PCR⿿

䎾䧴ざ䧭痦♧ꝆcDNAկ

M-MLV鷟鲮䔶ꃍ

䒸暟ծ䱲ꛏ

⿿鲮䔶

ざ䧭cDNA

RT-qPCR

䎾欽 fl

‡

‡

‡

鷟鲮䔶ꃍ掚珘㹁䚍㖈37°C傞崞䚍⢕

〳欽✵ⵖ㢊Ɤ鴪7kb涸痦♧ꝆcDNA

暵挿 :

‡

‡

䚍餘

朐䙖巊⡤

괃蒀偽蒀

鵘鳕倰䒭䎁ⱺ

⥂㶸庛䏞-20°C

餗〿A4464

Enhanced Avian Reverse Transcriptase 〳㼜 RNA 鲮䔶

⚹ DNAず傞儑衼䲿⼮ mRNA 垷匣ざ䧭 cDNA 涸佪桧կ鵯僽

歋✵劥妴㟞䔂㘗 AMV-RT 腊㢿鷟鲮䔶ꟋꝆ mRNA 垷匣腊㢿

剣佪瑬馊㢕勇涸✳紩絕匬〳⟄鸑鵂♧姿岁 RT-PCR ⿿䎾唬崵

⡛⚪䏞 mRNAկ

eAMV鷟鲮䔶ꃍ

䎾欽 fl

㼆⡛⚪䏞mRNAⰨ剣刿넞涸抳併䏞

㖈鳅넞庛䏞넞鴪 65°C♴⟄⠏葻涸鲮䔶腊⸂䩧

䒓 RNA ✳紩絕匬

剣佪ざ䧭ⰌꟋcDNAꟋ䏞〳鴪14.1kb

䚍腊 :

‡

‡

‡

㛇劥涸 PCR 䒸暟 ,SYBR@Green 䒸暟 , 䛎庛䪈㟞䒸暟 , 崵䎸䒸暟瘝կ

㹁ⵖ DNA 㻑呍薗ꃑ

鷓欽✵ 呍ꃑꃍ霚낉涸⿽叻雵䱲ꛏկ

㹁ⵖ qPCR 䱲ꛏ

ざ䧭ꆀ

紱⻊倰岁

䎸⴩Ɤ䏞

⥝껑

錞呔

0.21.010.0 & 15.0 µmol

膴渮ㄤ넞佪巊湱蒀靶HPLC

10-70 焦㛇刿Ɤ晙媯꨽ㅐ霧

5’熧ꃑ⻊

霚盗鄳䎁磋䧴很巊

䪮助錞呔

侨ꆀ

紱⻊倰岁

䎸⴩Ɤ䏞

⥝껑

錞呔

1 3 5 @ 10 OD

넞佪巊湱蒀靶HPLC

15-40 焦㛇刿Ɤ晙媯꨽ㅐ霧

䫣デ㛇㔙 [6-FAM™HEX™JOE™TAMRA™

ROX™& Cyanine 5]

帳抣㛇㔙 [TAMRA™ & Onyx Quencher™]

ꞏ呍ꃑ [Locked Nucleic Acid®LNA®]

霚盗鄳䎁磋

䪮助錞呔

㛇✵DNAㄤRNA涸ⴔ㶩霏倗꨽銳劢⥝껑䧴叻雵涸㻑呍薗ꃑ

랾⯘偫♴Sigma-Aldrich㖈㹁ⵖ㻑呍薗ꃑざ䧭剣满馄鵂30䎃

涸⚪㺢絑낉䧮⟌涸㹁ⵖ⻊剪⸉〳⟄➢♧䒓㨤㽠䲿⣘㻑呍薗

ꃑ⚁㹻涸霫絈䪮助䒊雳⚹ⴔ㶩霏倗ⵖ鸣㉁䲿⣘⚁⚌佅䭯կ

12

LNA ꝉ呍ꃑ僽♧猫倝㘗涸呍ꃑ碫⡃暟⺫ゎ 2'-O4'-C ❇歌㛇咕կ霪咕ꝉ㹁㖈 3'-C 匬韌⚥ꣳⵖ✫ト㊢呍祪梠涸厫꯸䚍䎇㼜絕匬

ꝉ㹁⚹⴮䚍涸⿽梠䕎䒭կ鵯〳⟄䲿넞掚珘㹁䚍䎇㟞⸈䎾欽薴㔵կ

LNA

Basic PCR primers

㛇炄PCR䒸暟

SYBR® Green䒸暟

⿽叻䱲ꛏ

ⴔ㶩⥌叻

LightCycler®䱲ꛏ

Scorpions®䱲ꛏ

ꝉ呍ꃑ〳欽✵⟄♴䒸暟䧴䱲ꛏ

‡

‡

‡

‡

‡

‡

‡

SNP唬崵

瘝⡙㛇㔔ꊹⵆ

氻⾲⡤唬崵

㢴ꅾ唬崵

氻嫫鲿ꆀ崵ꆀ

㛇㔔邍鴪ⴔ區

㛇㔔䭡餍唬崵

鷓欽✵⟄♴䎾欽

‡

‡

‡

‡

‡

‡

‡

ざ䧭ꆀ

紱⻊倰岁

䎸⴩Ɤ䏞

餘ꆀ䱽ⵖ

錞呔

剪⸉

0.05ծ0.2ծ1.0ծ10.0ㄤNjPRO

CartridgeծHPLCㄤ PAGE

7-15⚡焦㛇 100ꝉ㹁焦㛇

16-25⚡焦㛇 60ꝉ㹁焦㛇

26-40⚡焦㛇 40ꝉ㹁焦㛇

ㄤ41-60⚡焦㛇 20

ꝉ㹁焦㛇

100%餘靶

盗鄳䎁斕䧴巊⡤䕎䒭

崽䏞/⡤獤䔱♧⻊幊ざㄤⴔ鄳

PCR 䒸暟⺫䭍 SYBR®GreenㄤⰦ➭碫㘗涸㻑呍薗ꃑ

qPCR 䱲ꛏ

唬崵䱲ꛏ

ㄤ侨ꆀ

紱⻊倰岁

䎸⴩Ɤ䏞

厩俱

餘ꆀ䱽ⵖ

Format

剪⸉

⿽叻䱲ꛏ1ծ3ծ5 ㄤ 10 ODⴔ㶩⥌叻1ծ3ծ5 ㄤ 10 ODLightCycler® 䱲ꛏ0.1ծ0.25ծ1.5 ㄤ 15 OD⟄⿺

Scorpions® 䱲ꛏ1ծ5 ㄤ 10 OD

HPLC

15-40 ⚡焦㛇

FAMծHEXծROX ㄤ Cy3 瘝䫣デ㛇㔙ㄤ帳抣㛇㔙

100% 餘靶

䎁磋鄳✵楉梵蒀盗鼙⯕

崽䏞 / ⡤獤䔱♧⻊幊ざㄤⴔ鄳

⿿⛐㻑呍薗ꃑ

siRNA

Capture probes

䯲蜦䱲ꛏ

Aptamers

鵮〳欽✵⟄♴䎸⴩

‡

‡

‡

‡

‡

Ⱘ剣 1$3 䒸暟ծR1$3 䱲ꛏ⟄⿺Ⱖ➭碫㘗涸㻑呍薗ꃑ䕎䒭

13

Structure of Locked Nucleic Acid and native-state DNA monomers

HO

OH

Base

O

O

Locked Nucleic Acid Monomer

HO

OH

Base

O

DNA Monomer

Ugozzoli L, et al, Anal Biochem, 324, 143 – 152

覰蒀⢪欽ꝉ呍ꃑ瑲」䱲ꛏ Ⱘ剣3⚡ꝉ㹁焦㛇涸16翸⡤

ⴔ區瑲」㘗

DNAկ綁蒀⢪欽㣔搬DNA瑲」䱲ꛏ 25翸⡤

ⴔ區瑲」㘗DNAկ紤蒀⢪欽㣔搬DNA瑲」䱲ꛏ 25翸⡤

ⴔ區ꅿ欰㘗DNAկ稘

蒀⢪欽ꝉ呍ꃑ瑲」䱲ꛏ Ⱘ剣3⚡ꝉ㹁焦㛇涸16翸⡤

ⴔ區ꅿ欰㘗DNAկ

ꝉ呍ꃑ㻑呍薗ꃑⰨ剣佖㊤涸勇❜暵䚍㔔姼ⰦꟋ䏞刿瀊կⰨ⡤罜鎊♸Ɤ䏞紨⚹30-40⚡焦㛇涸DNA㻑呍薗ꃑ湱嫱Ɤ䏞⚹

20-30⚡焦㛇涸ꝉ呍ꃑ㻑呍薗ꃑ腊㢿唬崵ꦼ⟄ⴔ區涸ꬶ叻䎸⴩㥵㺢ゎAT䧴GC涸⼓㚖կ

刿ⲥ烁涸㛇㔔㹁ꆀㄤ瘝⡙㛇㔔ꊹⵆ

⚹ꦼ⟄ⴔ區涸ꬶ叻䎸⴩䲿⣘刿皍⽀ծ刿抳崞涸霃雦

14

䎸⴩ ꝉ㹁焦㛇 5N þ5N þ5N ꝉ㹁焦㛇

GTGATATGC

GTGATATGC

GTGATATGC

0

3

9

29 °C

44 °C

64 °C

—

+5 °C

+3.9 °C

15 °C

35 °C

♸㣔搬DNA㻑呍薗ꃑ湱嫱㖈⚥瘝渮勵⟝♴嫦妃ꝉ㹁呍ꃑ⽀⡤》➿た㻑呍薗ꃑ♸Ⱖꬶ叻涸佖葻勇❜㖲〳㼜鍒Ꝇ庛䏞 Tm

䲿넞

㢴鴪8°Cկ㟞⸈㻑呍薗ꃑ⚥涸ꝉ呍ꃑ焦㛇侨ꆀ⠔䲿넞Tmկ

⠏挿

䲿넞掚珘㹁䚍ㄤ勇❜暵䒗䚍

cycle

1100

100

900

800

700

600

500

400

300

200

100

0

-100

1100

100

900

800

700

600

500

400

300

200

100

0

-100

0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50 52

FLuorescence units

㖈4/1㛇㔔ⴔ㘗ⴔ區⚥ꝉ呍ꃑ⿽叻雵䱲ꛏ涸ꊹⵆ腊⸂⠏✵%/\"⿽叻雵䱲ꛏ

餗〿 ❡ㅷ䲽鶣

B0300

D8418

F9037

G5516

76234

M5904

D8276

R7393

R2502

R6501

A7721

A6410

G6751

12032937001

UDGHL-RO

11444646001

D0184

欫蝲焦

DMSO

歌ꃀ腂

欥屘

瀖蹙

瀤暟屘

Klenow晙媯

RNAꃍ䫈ⵖ⵫

RNAꃍ䫈ⵖ⵫

Rnase H

Taqꃍ䫒⡤

♲熧ꃑ艑薗⿽熧ꃑꃍ

I㘗熧ꃑ欥屘膴孲ꃍ

Taqꃍ Roche

UNGꃍ

UNGꃍ

dUTP

Ⱖ➭ 1$3 㟞䔂⵫

ThermaStop™僽♧猫〳鷟掚゗⸓霚⵫幑⸈⵫僽♧猫⴯倝

涸掚゗⸓ㄤⲇ⨢姺霚⵫〳㟞䔂Taq DNA翸ざꃍ暵䒗䚍䎇䲿

넞PCR❡暟❡ꆀ♸掚珘㹁DNA翸ざꃍ湱絕ざկ㸐湬䱹⡲欽✵

翸ざꃍ⟄꣈姺⡛✵50°C涸ꬋ暵䒗䚍ꃍ崞䚍կ翸ざꃍ崞䚍㖈

60˫㸤Ⰼ䛪㢕⡎鸑鵂ⲇ⽲⿿䎾鄄ThermaStop™ⱄ妃䫈ⵖկ

㼜ThermaStop™幑⸈ⵌPCR⿿䎾⚥〳䲿넞抳併䏞ㄤ䚪❡ꆀկ

PCR幑⸈⵫

7KHUPD6WRSŒ

ThermaGo™僽絑鵂⥝껑涸呍ꃑThermaGo™幑⸈⵫〳鸑

鵂PCR㟞䔂Taq DNA翸ざꃍ暵䒗䚍♸ThermaStop™幑⸈

⵫⼸ず⡲欽䲿넞Taq⥂溫䏞〳䫈ⵖPCR鷍抠ㄤ䒁⠽姿낢⚥涸

ꝡ霴䒸〄կ㸐䲿넞✫㹊傞ㄤ絊挿ⴔ區涸〳ꅾ㢕䚍կ

ThermaStop™霚 ⵫ 㖈 䪈 㟞 ⵸ た 饰 ⡲ 欽ThermaGo™㖈

PCR 涸」䚍鷍抠ㄤ䒁⠽姿낢⚥饰⡲欽կ

7KHUPD*RŒ

㕃2. ThermaSTOP-RT™腊僈儑䲿넞鷟鲮䔶涸暵䒗䚍

ꅾ銳䲿爙

1. 䧮⟌䒊雳 ThermaStop™ㄤ ThermaGo™ず傞⢪欽

2.ThermalSTOP-RT™♶〳♸ ThermaStop™ㄤ

ThermaGo™ず傞⢪欽

㕃1. 幑⸈⵫㼆♧姿岁ㄤ⚙姿岁涸RT-PCR鿪剣欽♧姿岁佪卓刿僈儑

ThermaSTOP™腊僈儑䲿넞掚゗⸓Taqꃍ涸暵䒗䚍ㄤ抳併䏞

Ⱖ腊㢿㖈⡛庛♴♸鷟鲮䔶ꃍ湱✽⡲欽➢罜⟄Ⲹ㼱㼋荝ꬋ暵

䒗䚍❡暟涸ꝡ霴䒸〄䲿넞唬崵抳併䏞⟄⿺䲿넞暵㹁D%/\"

❡ꆀ䲿넞넞䏞㢴ꅾ351$3ⴔ區涸ⲥ烁䚍⯈雽㼆䗎ꆀ宐䎂

涸3/\"鵳遤ⲥ烁涸㹁ꆀ崵㹁կ

7KHUPD672357Œ

Correct Product

A B C D E A B C D E

M TS TS TS TS TS

Hot Start DNA Polymerases

M = Low Range DNA Size Marker

Starting Material: 60 pg Human Genomic DNA

= No ThermaStop™ TS = ThermaStop™ added

15

16

餗〿 ❡ㅷ䲽鶣

780502

780550

780540

780546

781327

781366

781368

781373

781364

781391

781605

732022

732024

732028

732032

732724

732726

732728

732730

732732

732734

#SBOE 署勞

flSRڳ୏ख़ڶm঒խत

flSRڳ୏ख़ڶm঒խत

तֺ঒ڠҶԆ؍m঒ڶख़୏ڳSfl

तֺ঒ڠҶԆ؍m঒ڶख़୏ڳSR

ҷ৻঒঒तুՠ੸ͫॳत

fiffi؀6)8ߡmޗ娕ଆͫѺؠ

fiffi؀6)8ߡmޗ娕ଆͫ߶ӕ

fiffi؀6)8ߡmԡ娕ଆͫѺؠ

fiffi؀6)8ߡग਩Ѻؠऀй2OMNZ)_IRKX®ؔ୏6)8њ

ߡدਔͫऀй8KGR:OSK6)8

(8'4*VRGZKY®ڳ؀ߡffifim؀

਩ޗmvRͫף⩾֘१䃠੸ށ

਩ޗmvRͫflף⩾֘१䃠੸ށ

ށ੸१䃠֘⩽ףmvRͫ௰਩ͫ

਩੒mvRͫflף⩾֘१䃠੸ށ

:OV(U^⩽ףढͫflvRͫ(/5)+8:®ࢯ唾

:OV(U^⩽ףढͫvRͫ(/5)+8:®ࢯ唾

:OV(U^⩽ףढͫvRͫ(/5)+8:®ࢯ唾

:OV(U^⩽ףढͫflvRͫ(/5)+8:®ࢯ唾

:OV(U^⩽ףढͫflvRͫ(/5)+8:®ࢯ唾

:OV(U^⩽ףढͫflvRͫ(/5)+8:®ࢯ唾

餗〿 ❡ㅷ䲽鶣

AXYPCR96M2HSC

AXYPCR96C

AXYAM96PCRRD

AXYPCR0208C

AXYPCR02CPC

AXYPCR2CPRTC

AXYMCT150C

AXYMCT200O

AXYMCT200C

AXYT300

AXYT300R

AXYT300RS

AXYT200Y

AXYT200YRS

AXYTF1000

AXYTF1000RS

AXYTF1000LRS

PCR匣

PCR匣

PCR匣㼓芘

PCR Ⱃ翫盗

Ⱃ翫盗渷

PCR盗渷

1.5ml猌䗱盗

2ml猌䗱盗

2ml猌䗱盗

0.5-10ul卦㣢

0.5-10ul卦㣢

0.5-10ul卦㣢

200ul卦㣢

200ul卦㣢

1ml卦㣢

1ml卦㣢

1ml卦㣢

$PSOJOH® 署勞

17

KK4600

KK4601

KK4602

KK4603

KK4604

KK4605

KK4606

KK4607

KK4608

KK4609

KK4610

KK4611

KK4701

KK4702

KK4703

KK4715

KK4705

KK4706

KK4707

KK4714

KK4709

KK4710

KK4711

KK4716

KK4717

KK4718

KK5603

KK5601

KK5608

KK5609

KK5101

KK5102

KK5103

KK4650

KK4651

KK4652

KK4660

KK4661

KK4662

KK4670

KK4671

KK4672

KK4680

KK4681

1X1ml

1X5ml

2X5ml

1X1ml

1X5ml

2X5ml

1X1ml

1X5ml

2X5ml

1X1ml

1X5ml

2X5ml

1ml

5ml

10ml

50ml

1ml

5ml

10ml

50ml

1ml

5ml

10ml

1ml

5ml

10ml

100rxns

250rxns

1.25ml

6.25ml

1.25ml

6.25ml

25ml

1X1ml

1X5ml

2X5ml

1X1ml

1X5ml

2X5ml

1X1ml

1X5ml

2X5ml

1X1ml

1X5ml

KAPA SYBR FAST, Universal

KAPA SYBR FAST, Universal

KAPA SYBR FAST, Universal

KAPA SYBR FAST, ABI Prism

KAPA SYBR FAST, ABI Prism

KAPA SYBR FAST, ABI Prism

KAPA SYBR FAST, Bio-Rad iCycle™

KAPA SYBR FAST, Bio-Rad iCycle™

KAPA SYBR FAST, Bio-Rad iCycle™

KAPA SYBR FAST, for Roche LightCycler® 480

KAPA SYBR FAST, for Roche LightCycler® 480

KAPA SYBR FAST, for Roche LightCycler® 480

KAPA Probe Fast Uni

KAPA Probe Fast Uni

KAPA Probe Fast Uni

KAPA Probe Fast Uni

KAPA Probe Fast ABI

KAPA Probe Fast ABI

KAPA Probe Fast ABI

KAPA Probe Fast ABI

KAPA Probe Fast BioRad

KAPA Probe Fast BioRad

KAPA Probe Fast BioRad

KAPA Probe Fast ROX Low

KAPA Probe Fast ROX Low

KAPA Probe Fast ROX Low

KAPA2G Fast HotStart ReadyMix

KAPA2G Fast HotStart ReadyMix

KAPA2G Fast HotStart ReadyMix with dye

KAPA2G Fast HotStart ReadyMix with dye

KAPA2G Fast ReadyMix

KAPA2G Fast ReadyMix

KAPA2G Fast ReadyMix

KAPA SYBR FAST One-Step Universal

KAPA SYBR FAST One-Step Universal

KAPA SYBR FAST One-Step Universal

KAPA SYBR FAST One-Step ABI

KAPA SYBR FAST One-Step ABI

KAPA SYBR FAST One-Step ABI

KAPA SYBR FAST One-Step Bio-Rad iCycler

KAPA SYBR FAST One-Step Bio-Rad iCycler

KAPA SYBR FAST One-Step Bio-Rad iCycler

KAPA SYBR FAST One-Step for Roche LightCycler

KAPA SYBR FAST One-Step for Roche LightCycler

,\"1\" 霚⵫渱❡ㅷ⴩邍

餗〿 ❡ㅷ䲽鶣 錞呔

♸呍ꃑ⻊㷖곭㚖Ⱆ雩涸Ⰼ椕⠑⠶ざ⡲䝠㼜⠔刿荈⥌ծ刿剣

䪾䳣㖑ざ䧭DNAㄤRNA㻑呍薗ꃑկ偽雿䝠꨽銳㣐䪠ꆀ涸叻ⲥ

❇熧ꃀ腂⽀⡤鵮僽暵婋⽀⡤䧮⟌鿪荝⸂✵⚹䝠䲿⣘⚡䚍⻊

涸鍒Ɀ倰呩կ䧮⟌䭆剣ぐ碫⚁欽㹊낉㹔⟄⿺䊨蒌䒓〄ㄤ䪈㣐

⻊倰꬗涸䧭擿⚁⚌濼霋〳⟄䌐⸔䝠䎾㼆♶倗」⻊涸䋑㖞梠

㞯䨾䌄勻涸䎾欽ㄤ佪桧䮋䧶կ

❇熧ꃀ腂⽀⡤ㄤ㔿湱〳䱽㶰䖈梯槴(CPG)㖈䧮⟌⡙✵䗞㕂宪

㜃涸㛇㖑欰❡կ

鹫25䎃涸呍薗ㄤ❇熧ꃀ腂欰❡⚁⚌濼霋ㄤ杝暵涸䪮助濼霋

鳇⟄⚁⚌〳ꬑ涸고湡盗椚䪮腊雮䝠➢⚥「渤կ

䧮⟌䲿⣘〳忘駈Ⳝ⛖⟣⡦ざ䧭⟉ ⺫䭍ABI™394ծABI™

3900ծExpedite™ծAKTA®ㄤMerMade

涸⺫鄳⟄⿺㹁ⵖ

⺫鄳կ䧮⟌鵮䲿⣘ぐ猫㽯㼄涸巊⡤扪鄳ㄤ幊ざ⺫鄳Ղ➢霚⵫

櫕ⵌ1,400L〳㔐佐㺂㐼կ

18

N

HN

O

O

⥝껑烿徃㹁ⵖ

O

HO

OH

焦㛇

⥝껑MOETOM㹁ⵖ

O

HO

OH

OH

OH

Base

HO

O

N

HN

O

O

OH

HO

O

㹁ⵖ⺫鄳

㔔⚹⚁⚌䨾⟄馄馊

䧮⟌涸䪮助⚁Ɤ

‡呍薗ꃑ

‡叻ⲥ⥂䫡ㄤ䘯鸟膴⥂䫡❇熧ꃀ腂

‡㔿湱ざ䧭佅䭯暟

‡ざ䧭叙

‡暵婋❇熧ꃀ腂

‡叻雵暟/厩俱

‡❜翫⵫

‡霚⵫ㄤ很⵫

‡ⵖ㢊蒀靶

/䎃

雩霆玐䎸

‡〳㹊梡⯘紩荛100⼪⯘錞垷涸ⵖ㢊蒀靶

‡❇熧ꃀ腂❡腊馄鵂5Ⱆょ

‡㖈呍ꃑ❡ㅷㄤ暵婋⽀⡤倰꬗⚪㺢絑낉涸猰㷖㹻䲿⣘䪮助佅䭯

‡呍ꃑざ䧭ぐ⚡倰꬗涸䊨蒌䒓〄⚁⚌濼霋

‡腊⿺傞朐䙖刿倝ㄤ고湡騈驶涸⚁欽고湡盗椚䪮助

‡餘ꆀ⥂霆–黽䗄ISO-9001

‡巊⡤㢅椚ㄤ䊨蒌涸䪮助佅䭯

㼊宠鷓ざ䝠涸⚡䚍⻊鍒Ɀ倰呩

3UROLJRŒ霚⵫

19

〳欽䪮助爙⢾

䊨蒌/䪮助 暵挿 ⿿䎾/爙⢾

熧ꃀ腂⻊ ✳䒗⚋㛇ǃ宊㛇⛩㛇❇熧ꃀ腂㛇㔙涸䒸Ⰶ R O P

N

O

C

N

R O H

♲薅歌㛇✳歌孻㛇♲歌㛇⻊ ♲薅㛇歌㛇⥂䫡㛇涸䒸Ⰶ R OH R O C OCH3

OCH3

歌炊掬㛇⻊/⿡歌炊掬㛇⻊ 歌炊掬㛇⥂䫡㛇涸䒸Ⰶ䧴⿡ꤑ

焦㛇

O

O OH

O

Si

Si

O

O

HO

HO

OH

焦㛇

N-ㄤO-ꃀ⻊ 呍焦㛇ㄤ呍祪⥂䫡㛇涸䒸Ⰶ

N

O N

NH2

呍祪

呍祪

N

O N

NH

C

R

O

呍焦㛇鲮⻊ 僒唬崵涸呍薗ぢⰦ➭焦㛇涸呍薗鲮⻊

HN

O N

O

祪

CH3 N

O N

NH2

CH3

祪

邍꬗⻊㷖 ⸆腊⻊梯槴ㄤ剣劼翸ざ暟邍꬗涸⸈鲿ㄤ⸈䌨

N

H

O

O

O

O

O R

NH2

焦㛇

ⵖ㢊蒀靶 㖈姻湱炊脸♳鵳遤넞⾓Ⰼ荈⸓ⴔ猌

⺫䭍〳荈歋玐䱽涸唑䏞峤膴

ⴔꛦ

紱䏞
>99%

磦ざ䧭

❡ㅷ

0.00 0.00

0.20

0.40

0.60

0.80

1.00

5.00 10.00 15.00 20.00 25.00 30.00

AU

霃㢊

‡➢㹊낉㹔錞垷ⵌ㉁⚌錞垷涸CPGⵖ⡲❡ㅷ絁

‡荈⸓ⵖ㢊㘗HPLC䊨蒌錞垷剒㣐〳鴪ⵌ500 mm涸蒀靶叙湬䖈

‡⚁꡶涸⻊㷖ㄤ䊨蒌䪮助䒓〄㹊낉㹔

‡ⴔ區腊⸂NMRծIRծUV/VISծ蚃⯕ㄤMS⯕靶ծHPLCծGC

PCR/qPCR 03/20 V01

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