— PCR/qPCR产品手册

技术领先，所以表现不凡

• 反应速度快

• 合成能力强

• 兼容样本广

• 灵敏度高

分子生物学试剂市场基本上都是提供野生

型的 DNA 聚合酶。随着客户实验要求的提

升和生物样本复杂性的提高，野生型 DNA

聚合酶的局限性在许多实验中也逐渐凸显

出来，如扩增效率低，特异性差，高 AT 或

GC 含量样本扩增结果差，样本中或 PCR 系

统中有些抑制剂对反应有干扰等等。

自 2017 年 1 月 1 日起，默克成为罗氏旗

下 KAPA Biosystems 产品的独家供应商，

在美国、巴西和日本以外的所有国家提供聚

合酶链反应 (PCR) 和实时定量聚合酶链反

应（qPCR）所用的全新酶及试剂盒产品。

KAPA Biosystems 的 PCR 和 qPCR 产品

在实验中的优异表现主要源自它提供的

DNA 聚合酶是通过 Directed Evolution

工程化修饰的。野生型 DNA 聚合酶基因经

过多轮的突变，筛选和扩增，最终获得客户

需要的特性，如增加酶的合成能力或降低酶

对于 SYBR Green 染料的敏感性等。该技

术同样适用于 RNA 修饰酶的优化。

PCR

QPCR

Taq酶及相

关产品

2G酶及相

关产品

SYBR

荧光定量

PROBE

Mouse

Genotyping

相关产品

DNA快速提

取及扩增

荧光定量

2

KAPA2G FAST

DNA Polymerase

Total PCR times for the generation of different sized amplicons for

human genomic DNA (≤3.5 kb) and lambda (≤5 kb) using wild-type

Taq DNA polymerase and KAPA2G Fast DNA Polymerase.

The amount of time that can be saved using protocols based on wild-type

Taq is limited by the extension rate of the enzyme. KAPA2G Fast DNA Polymerase is based on a secondgeneration polymerase with an ability to synthesize DNA faster than wild-type Taq or other DNA polymerases. Reaction

times are based on a 35-cycle program using the cycling profile recommended by each kit manufacturer.

实例一 扩增时间减少 75%，1 sec/kb KAPA2G Fast DNA Polymerase 是通过

野生型Taq酶改造的第二代工程酶，具有

更高的合成能力和合成速度。

Amplification of 5 human gene fragments using KAPA2G Fast HotStart or competitor hot-start Taq formulations.

Reactions (25 μL) contained 5 ng human genomic DNA and 0.5 units (KAPA2G Fast HotStart and Competitor I) or 0.625 units (Competitor A and Q)

enzyme. For amplicons with a GC content >65% (lanes 2 and 3), 7.5%

DMSO was included in reactions. A 3-step cycling profile (35 cycles) with 15

sec denaturation (95˚C) and 15 sec annealing (60˚C) per cycles was used

for all enzymes. The extension (72˚C) was 1 sec/cycle for KAPA2G Fast HotStart and 60 sec/cycle for competitor enzymes. The total reaction time for

each enzyme is indicated.

实例二 更快的扩增速度，更好的结果

产品列表

货号 产品描述 产品规格

产品特点 :

反应更快

PCR反应时间缩短75%。

合成能力更强

延伸时间缩短至1 sec/kb。

兼容更广

兼容多种实验样本，甚至富含AT-和

GC-的样本。

灵敏度及产量更高。

第一个兼具扩增速度与灵敏度的 DNA 聚合酶

KK5020

KK5021

KK5008

KK5009

KK5523

KK5503

KK5501

KK5519

KK5530

KK5502

KK5500

KK5603

KK5601

KK5511

KK5512

KK5510

KK5513

KK5101

KK5608

KK5609

100 units

250 units

100 units

250 units

100 units

250 units

500 units

2500 units

100 units

250 units

500 units

100 rxns

250 rxns

500 units

250 units

500 units

250 units

1.25 mL

1.25 mL

6.25 mL

KAPA2G Fast

KAPA2G Fast

KAPA2G Fast + KAPA dNTP Mix

KAPA2G Fast + KAPA dNTP Mix

KAPA2G Fast HotStart

KAPA2G Fast HotStart

KAPA2G Fast HotStart

KAPA2G Fast HotStart

KAPA2G Fast HotStart + KAPA dNTP Mix

KAPA2G Fast HotStart + KAPA dNTP Mix

KAPA2G Fast HotStart + KAPA dNTP Mix

KAPA2G Fast HotStart Ready Mix

KAPA2G Fast HotStart Ready Mix

KAPA2G HotStart PCR Kit with Mg-free

Buffer M

KAPA2G HotStart PCR Kit with Mg-free

Buffer M, with dNTPs

KAPA2G HotStart PCR Kit with Mg-free

Buffer M, with dNTPs

KAPA2G HotStart PCR Kit with Mg-free

Buffer M

KAPA2G ReadyMix with dye

KAPA2G HotStart ReadyMix with dye

KAPA2G HotStart ReadyMix with dye

3

KAPA Mouse

Genotyping Kits

1小时鉴定基因编辑小鼠!

产品特点 :

仅一个试剂盒完成动物组织DNA抽提和扩增

DNA抽提试剂盒包含新型的热稳定蛋白酶和

缓冲液，15min快速抽提

二代DNA聚合酶45min完成PCR

高灵敏度，高特异性

高灵敏度 实例一 用推荐的标准流程PCR小鼠基因组DNA (1 kb)

高特异性 实例二 用推荐的标准流程PCR小鼠基因组DNA (2 kb),

at 50 °C and 60 °C 退火

KK7302

KK7351

KK5620

KK5621

Mouse Genotyping Kit

HS Mouse Genotyping Kit

2G Fast HotStart

Genotyping PCR Kit

2G Fast HotStart

Genotyping PCR Kit

500 rxn

100 rxn

100 rxn

500 rxn

产品列表

货号 产品描述 产品规格

Tail or ear tissue Incubation at Fast PCR Gel electrophoresis

75°C & 95°C

明星产品

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45

min

15

min

4

试剂盒中的DNA聚合酶对SYBR Green

I染料具有耐受性。尤其适合低拷贝数或

AT- 和GC-含量丰富的样本。

KAPA SYBR FAST qPCR Kits含有第一个通过工程化修饰对

SYBR Green I染料具有耐受性的DNA聚合酶。

KAPA SYBR®

FAST qPCR

KITS

产品特点 :

灵敏度更高

准确性更高

合成能力更强

使用快速PCR流程，可以

使实验时间缩短55%

尤其适用于高GC含量和

低拷贝数的检测样本的基

因表达分析

实例一 实例二 KAPA SYBR FAST qPCR Kits兼容广泛的

GC含量和扩增子长度，扩增效率上没有偏

好性。95 – 105%的高反应效率增加了反

应的准确性和重复性

Reaction Efficiency vs. GC content

Reaction Efficiency vs. Amplicon Length

No bias in amplification efficiency across a wide range of GC

contents (44.2 – 62.5%) or amplicon lengths (86 – 249 bp)

was observed with KAPA SYBR FAST.

Amplification efficiencies achieved for ten housekeeping genes with

the KAPA SYBR FAST Universal qPCR Kit were plotted against GC content (left) or amplicon length (right). The reaction efficiency achieved

for each of the ten genes fell within the optimal range of 95 – 105%,

independent of the nature or length of the amplicon.

Two target genes were amplified using a

10-fold dilution series of human genomic DNA

(16 ng to 1.6 pg per 20 μL reaction) comparing four commercial kits. KAPA SYBR FAST

consistently provides high performance for

accurate expression quantitation across a

range of difficult targets.

DMD (23.1% GC)

NOTCH (72.2% GC)

5

实例三 仅需40分钟即可完成Real-time PCR反应，

并且表现很好的实验结果

Amplification Plot

A 150 bp fragment of the human coagulation factor V gene was

amplified from a set of 5 10-fold dilutions of human genomic DNA

(20 ng - 2 pg) with a standardcycling protocol (green) and a

fast-cycling protocol (blue).

试剂盒主要应用

基因表达分析

芯片结果验证

低拷贝数样本检测

基因敲出验证

ChIP实验分析

二代测序文库定量

明星产品

产品列表

货号 产品描述 产品规格

KK4600 KAPA SYBR® FAST, Universal

KK4601 KAPA SYBR FAST, Universal

KK4602 KAPA SYBR FAST, Universal

KK4603 KAPA SYBR FAST, ABI Prism®

KK4604 KAPA SYBR FAST, ABI Prism

KK4605 KAPA SYBR FAST, ABI Prism

KK4606 KAPA SYBR FAST, Bio-Rad iCycler™

KK4607 KAPA SYBR FAST, Bio-Rad iCycler

KK4608 KAPA SYBR FAST, Bio-Rad iCycler

KK4609 KAPA SYBR FAST, for Roche

LightCycler® 480

KK4610 KAPA SYBR FAST, for Roche

LightCycler® 480

KK4611 KAPA SYBR FAST, for Roche

LightCycler® 480

1 x 1 mL

1 x 5 mL

2 x 5 mL

1 x 1 mL

1 x 5 mL

2 x 5 mL

1 x 1 mL

1 x 5 mL

2 x 5 mL

1 x 1 mL

1 x 5 mL

2 x 5 mL

6

KAPA SYBR FAST One-Step qRT-PCR Kits 是专门为qRT-PCR反应优化的试剂盒，

在灵敏度、特异性、反应效率和荧光值方面都有极佳的表现。试剂盒仅需一步，一个管

子，即可以完成RNA的快速定量，操作非常方便。

KAPA SYBR®

FAST ONE-STEP

qRT-PCR KITS

一步，一管，RNA快速定量

RRMI gene (94 bp, 45.7% GC) amplified from 10-fold dilution series of RNA (100 ng to 10 pg per

20 μL reaction) isolated from human placenta. KAPA SYBR FAST One- Step qRT-PCR Kit demonstrates earlier Cq values, higher fluorescence, and optimal reaction efficiencies.

MS2 bacteriophage RNA targets of increasing length were amplified at 0.8 pg per 20 μL reaction.

KAPA SYBR® FAST One-Step qRT-PCR Kit maintained high performance across all amplicon

lengths.

7

更高的扩增效率，更高的荧光值，更早的 Cq 值

适用于更广泛的目标基因长度Normalized fluorescence

Cycle

0 5 10 15 20 25 30 35 40

5 x 104

6 x 104

4 x 104

3 x 104

2 x 104

1 x 104

0

9 x 104

8 x 104

7 x 104

KAPA SYBR® FAST qRT-PCR

Competitor I

1 1 x 102 1 x 104 1 x 106

20

15

25

30

35

40

Competitor I

Slope: -3.207

KAPA SYBR® FAST qRT-PCR

Slope: -3.310

Efficiency: 1.00

Amount (copies)

Cq(cycle)

Normalized fluorescence

Cycle

0 5 10 15 20 25 30 35 40

5 x 104

6 x 104

4 x 104

3 x 104

2 x 104

1 x 104

0

9 x 104

8 x 104

7 x 104

KAPA SYBR® FAST qRT-PCR

Competitor Q

1 1.02 1.04 1.06

20

15

25

30

35

40

Competitor Q

Slope: -2.951

KAPA SYBR ® FAST qRT-PCR

Slope: -3.310

Efficiency: 1.00

Amount (copies)

Cq(cycle) Normalized fluorescence

Cycle

0 5 10 15 20 25 30 35 40

20

15

25

30

35

40

Competitor A

Slope: -2.298

Efficiency: 1.72

KAPA SYBR ® FAST qRT-PCR

Slope: -3.405

Efficiency: 0.97

Amount (copies)

KAPA SYBR ® FAST qRT-PCR

Competitor A

Cq(cycle)

5 x 104

6 x 104

4 x 104

3 x 104

2 x 104

1 x 104

0

9 x 104

8 x 104

7 x 104

1 1 x 102 1 x 104 1 x 106

Normalized fluorescence

0 5 10 15 20 25 30 35 40

5 x 104

6 x 104

4 x 104

3 x 104

2 x 104

1 x 104

0

9 x 104

8 x 104

7 x 104

162 bp

KAPA SYBR ® FAST

Competitor T

Competitor I

Competitor Q

Cycle Cycle

0 5 10 15 20 25 30 35 40

Normalized fluorescence 5 x 104

6 x 104

4 x 104

3 x 104

2 x 104

1 x 104

0

9 x 104

8 x 104

7 x 104

279 bp

KAPA SYBR® FAST

Competitor T

Competitor I

Competitor Q

Cycle

0 5 10 15 20 25 30 35 40

Normalized fluorescence 5 x 104

6 x 104

4 x 104

3 x 104

2 x 104

1 x 104

0

9 x 104

8 x 104

7 x 104

414 bp

KAPA SYBR ® FAST

Competitor T

Competitor I

Competitor Q

8

279 bp fragment amplified in replicates of 6 from a 10-fold dilution series of

commercial MS2 bacteriophage RNA (80 pg to 0.8 fg per 20 μL reaction).

Reaction efficiency of 95% calculated for all 6 replicates per data.

试剂盒主要应用

SNP 基因分型

基因表达分析

芯片结果验证

基因敲除验证

RNAi和miRNA研究

Normalized fluorescence

0 5 10 15 20 25 30 35 40

5 x 103

5 x 102

0

5 x 104

Slope: -3.430

Y intercept: 33.97

Efficiency: 0.95

R2: 0.999

Cycle

更高的灵敏度和重复性

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产品列表

货号 产品描述 产品规格

KK4650 KAPA SYBR® FAST One-Step Universal

KK4651 KAPA SYBR FAST One-Step Universal

KK4652 KAPA SYBR FAST One-Step Universal

KK4660 KAPA SYBR FAST One-Step ABI Prism®

KK4661 KAPA SYBR FAST One-Step ABI Prism

KK4662 KAPA SYBR FAST One-Step ABI Prism

KK4670 KAPA SYBR FAST One-Step Bio-Rad

iCycler™

KK4671 KAPA SYBR FAST One-Step Bio-Rad

iCycler

KK4672 KAPA SYBR FAST One-Step Bio-Rad

iCycler

KK4680 KAPA SYBR FAST One-Step for Roche

LightCycler®

KK4681 KAPA SYBR FAST One-Step for Roche

LightCycler®

1 x 1 mL

1 x 5 mL

2 x 5 mL

1 x 1 mL

1 x 5 mL

2 x 5 mL

1 x 1 mL

1 x 5 mL

2 x 5 mL

1 x 1 mL

1 x 5 mL

9

KK1007

KK1017

KK1041

KK7101

KK7102

KK7103

KK7100

KK7151

KK7152

KK4203

KK4201

KK4202

KK7351

KK7352

KK3601

KK3602

KK3501

KK3502

KK3503

KK3006

产品列表

货号 产品描述 规格 货号 产品描述 规格

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KK4662

KK4660

KK4661

KK4672

KK4670

KK4671

KK4680

KK4681

KK4652

KK4650

KK4651

KK4605

KK4603

KK4617

KK4604

KK4608

KK4606

KK4607

KK4611

KK4609

KK4610

KK4621

KK4619

KK4622

KK4620

KK4602

KK4600

KK4618

KK4601

BK1000

KK1014

BK1002

KK1015

KK3604

KK3605

KK3009

KK1513

KK1508

KK1510

KK1512

KK1509

KK1511

KK1006

KK1024

BK1001

KK1008

BK1003

KK1016

BK1005

KK1021

KAPA dNTP Kit 100 mM

KAPA dNTP Mix 10 mM

KAPA dNTP Mix 10 mM

KAPA Express Extract

KAPA Express Extract

KAPA Express Extract

KAPA Express Extract

KAPA Express Extract+RM

KAPA Express Extract+RM

KAPA HRM Fast

KAPA HRM Fast

KAPA HRM Fast

KAPA HS Mouse Genotyping

KAPA HS Mouse Genotyping

KAPA Long Range HS RM

KAPA Long Range HS RM

KAPA Long Range HS+dNTPs

KAPA Long Range HS+dNTPs

KAPA Long Range HS+dNTPs

KAPA Long Range+dNTPs

KAPA SYBR Fast 1step ABI

KAPA SYBR Fast 1step ABI

KAPA SYBR Fast 1step ABI

KAPA SYBR Fast 1step BioRad

KAPA SYBR Fast 1step BioRad

KAPA SYBR Fast 1step BioRad

KAPA SYBR Fast 1step LC480

KAPA SYBR Fast 1step LC480

KAPA SYBR Fast 1step Uni

KAPA SYBR Fast 1step Uni

KAPA SYBR Fast 1step Uni

KAPA SYBR Fast ABI

KAPA SYBR Fast ABI

KAPA SYBR Fast ABI

KAPA SYBR Fast ABI

KAPA SYBR Fast BioRad

KAPA SYBR Fast BioRad

KAPA SYBR Fast BioRad

KAPA SYBR Fast LC480

KAPA SYBR Fast LC480

KAPA SYBR Fast LC480

KAPA SYBR Fast ROX Low

KAPA SYBR Fast ROX Low

KAPA SYBR Fast ROX Low

KAPA SYBR Fast ROX Low

KAPA SYBR Fast Uni

KAPA SYBR Fast Uni

KAPA SYBR Fast Uni

KAPA SYBR Fast Uni

KAPA Taq

KAPA Taq

KAPA Taq

KAPA Taq

KAPA Taq EXtra HS RM

KAPA Taq EXtra HS RM

KAPA Taq EXtra+dNTPs

KAPA Taq HS

KAPA Taq HS

KAPA Taq HS

KAPA Taq HS no buffer

KAPA Taq HS+dNTPs

KAPA Taq HS+dNTPs

KAPA Taq RM

KAPA Taq RM+dye

KAPA Taq+dNTPs

KAPA Taq+dNTPs

KAPA Taq+dNTPs

KAPA Taq+dNTPs

KAPA Taq+dNTPs+dye

KAPA Taq+dNTPs+dye

0.25 mL ea

0.5 mL

5 mL

100 rxn

250 rxn

500 rxn

50 rxn

100 rxn

500 rxn

10 mL

1 mL

5 mL

100 rxn

500 rxn

1.25 mL

6.25 mL

100 U

250 U

500 U

100 U

1000 rxn

100 rxn

500 rxn

1000 rxn

100 rxn

500 rxn

100 rxn

500 rxn

1000 rxn

100 rxn

500 rxn

10 mL

1 mL

50 mL

5 mL

10 mL

1 mL

5 mL

10 mL

1 mL

5 mL

10 mL

1 mL

50 mL

5 mL

10 mL

1 mL

50 mL

5 mL

2500 U

250 U

5000 U

500 U

1.25 mL

6.25 mL

250 U

2500 U

250 U

500 U

2500 U

250 U

500 U

5×1.25 mL

5×1.25 mL

2500 U

250 U

5000 U

500 U

2500 U

250 U

KK3005 KAPA Long Range+dNTPs

KK7301 KAPA Mouse Genotyping

KK7302 KAPA Mouse Genotyping

KK4752 KAPA Probe Fast 1step Uni

KK4707 KAPA Probe Fast ABI

KK4705 KAPA Probe Fast ABI

KK4714 KAPA Probe Fast ABI

KK4706 KAPA Probe Fast ABI

KK4711 KAPA Probe Fast BioRad

KK4709 KAPA Probe Fast BioRad

KK4710 KAPA Probe Fast BioRad

KK4718 KAPA Probe Fast ROX Low

KK4716 KAPA Probe Fast ROX Low

KK4717 KAPA Probe Fast ROX Low

KK4703 KAPA Probe Fast Uni

KK4701 KAPA Probe Fast Uni

KK4715 KAPA Probe Fast Uni

KK4702 KAPA Probe Fast Uni

KK4300 KAPA Probe Force Uni

KK4301 KAPA Probe Force Uni

BK1007

KK1023

BK1004

KK1020

BK1006

KK1022

KK5020

KK5021

KK5121

KK5523

KK5519

KK5503

KK5501

KK5620

KK5621

KK5513

KK5511

KK5603

KK5601

KK5608

KK5609

KK5530

KK5502

KK5500

KK5512

KK5510

KK5801

KK5802

KK5101

KK5103

KK5102

KK5008

KK5009

KK5023

KK5024

KK5522

KK5525

KK5515

KK5517

KK5701

KK5702

KK5704

KK5705

KK5532

KK5516

KK5518

KK5004

KK5005

KK7251

KK7252

KAPA Taq+dNTPs+dye

KAPA Taq+dNTPs+dye

KAPA Taq+dye

KAPA Taq+dye

KAPA Taq+dye

KAPA Taq+dye

KAPA2G Fast

KAPA2G Fast

KAPA2G Fast Genotyping Mix

KAPA2G Fast HS

KAPA2G Fast HS

KAPA2G Fast HS

KAPA2G Fast HS

KAPA2G Fast HS Genotyping Mix

KAPA2G Fast HS Genotyping Mix

KAPA2G Fast HS Mg-free

KAPA2G Fast HS Mg-free

KAPA2G Fast HS RM

KAPA2G Fast HS RM

KAPA2G Fast HS RM+dye

KAPA2G Fast HS RM+dye

KAPA2G Fast HS+dNTPs

KAPA2G Fast HS+dNTPs

KAPA2G Fast HS+dNTPs

KAPA2G Fast HS+dNTPs Mg-free

KAPA2G Fast HS+dNTPs Mg-free

KAPA2G Fast Multiplex Mix

KAPA2G Fast Multiplex Mix

KAPA2G Fast RM

KAPA2G Fast RM

KAPA2G Fast RM

KAPA2G Fast+dNTPs

KAPA2G Fast+dNTPs

KAPA2G Robust

KAPA2G Robust

KAPA2G Robust HS

KAPA2G Robust HS

KAPA2G Robust HS

KAPA2G Robust HS

KAPA2G Robust HS RM

KAPA2G Robust HS RM

KAPA2G Robust HS RM+dye

KAPA2G Robust HS RM+dye

KAPA2G Robust HS+dNTPs

KAPA2G Robust HS+dNTPs

KAPA2G Robust HS+dNTPs

KAPA2G Robust+dNTPs

KAPA2G Robust+dNTPs

KAPA3G Plant HS

KAPA3G Plant HS

250 U

100 rxn

500 rxn

500 rxn

10 mL

1 mL

50 mL

5 mL

10 mL

1 mL

5 mL

10 mL

1 mL

5 mL

10 mL

1 mL

50 mL

5 mL

1 mL

5 mL

5000 U

500 U

2500 U

250 U

5000 U

500 U

100 U

250 U

6.25 mL

100 U

2500 U

250 U

500 U

1.25 mL

6.25 mL

250 U

500 U

1.25 mL

6.25 mL

1.25 mL

6.25 mL

100 U

250 U

500 U

250 U

500 U

1.25 mL

6.25 mL

1.25 mL

25 mL

6.25 mL

100 U

250 U

100 U

250 U

100 U

2500 U

250 U

500 U

1.25 mL

6.25 mL

1.25 mL

6.25 mL

100 U

250 U

500 U

100 U

250 U

250 U

500 U

Enzyme Selection Guide Product KA

PA

SYBR®FAST

KA

PA

P

ROBE FAST

KA

PA

HRM FAST

KA

PA Blood KA

PA3G Plant KA

PA2G Fast KA

PA2G Robust KA

PA HiFi/ HiFi Uracil+ KA

PA

Long Range

KA

PA

Taq

Performance

Sensitivity •••• •••• •••• •• ••• ••• •••• ••• •••• ••

Fidelity (vs Wild-type Taq) 1x 1x 1x 1x 3x – 4x 1x 1x 100x 3x – 4x 1x

•••• •••• •••• ••• ••• ••• ••• ••• •••• ••

Speed 20 sec ann/ext ≥20 sec ann/ext 30 sec ann/ext 60 sec/kb ext 30 sec/kb ext 1 – 15 sec/kb ext 15 – 60 sec/kb ext 15 – 60 sec/kb ext 60 sec/kb ext 60 sec/kb ext

Robustness •••• ••• ••• ••• •••• ••• •••• ••• ••• ••

Yield •••• •••• •••• ••• ••• ••• •••• •••• •••• ••

Application

qPCR

√

√

√

√

Routine PCR

√

√

√

√

√

√

√

Fast PCR/qPCR

√

√

√

√

√

√

√

Cloning

√

√

Colony PCR

√

√

Crude-sample PCR

√

√

√

√

GC-richPCR√√√√√√Specificity High-fidelity PCR

GCrich PCR

√

√

√

√

√

√

Gene expression

√

√

Genotyping

√

√

√

√

√

√

√

√

√

√

Long-range PCR

√

√

Multiplex PCR

√

√

√

√

√

√

NGS

√

√

√

√

√

Whole-blood PCR

√

Site-directed mutagenesis √

SNP detection

√

√

Amplicon size from different templates

Complex DNA targets

(human, mouse, etc.) 100 – 1500 bp 100 – 1500 bp 40 – 200 bp <3.5 kb <5 kb (plant) <3.5 kb <3.5 kb <15 kb <20 kb <3.5 kb

Less-complex DNA (bacteria,

plasmid, lambda, etc.) 100 – 1500 bp 100 – 1500 bp 40 – 200 bp N/A N/A <5 kb <5 kb <20 kb <20 kb <5 kb

cDNA 100 – 1500 bp 100 – 1500 bp 40 – 200 bp N/A N/A <3.5 kb <3.5 kb <15 kb <10 kb <3.5 kb

Additional features

3'-A product end • • • • • • • • •

Blunt product end •

5'–3' polymerase activity ••• • ••• •• •• ••• •• •• • •

5'–3' exonuclease activity • ••• • • • • • ••• •••

3'–5' exonuclease (proofreading) activity • ••• •

HotStart Available Standard Standard Standard N/A Standard • • HiFi: • HiFi U+: Standard • •

Strand displacement √ =Suitable for application

• =Relative activity

更多详情，敬请登陆：www.merckmillipore.com

技术服务电话：400 889 1988 中国技术服务中心：asiatechserv@merckgroup.com

更多详情，敬请登陆：www.merckmillipore.com 技术服务电话：400 889 1988 中国技术服务中心：asiatechserv@merckgroup.com

PCR/qPCR 03/17 V01

更多详情，敬请登陆：www.merckmillipore.com 技术服务电话：400 889 1988 中国技术服务中心：asiatechserv@merckgroup.com

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